Am. J. Respir. Crit. Care Med.,
Volume 161, Number 2, February 2000, 391-398
Differential Metabolism of Arachidonic Acid in
Nasal Polyp Epithelial Cells Cultured from
Aspirin-sensitive and Aspirin-tolerant Patients
MAREK L.
KOWALSKI,
RAFAL
PAWLICZAK,
JANINA
WOZNIAK,
KATARZYNA
SIUDA,
MARITA
PONIATOWSKA,
JOLANTA
IWASZKIEWICZ,
TOMASZ
KORNATOWSKI,
and
MICHAEL A.
KALINER
Department of Clinical Immunology and Allergy, and Department of Otorhinolaryngology, Medical University, Lodz, Poland;
and Institute of Asthma and Allergy, Washington, DC
The mechanism of aspirin (acetylsalicylic acid [ASA]) sensitivity associated with severe asthma and
chronic rhinosinusitis with nasal polyps ("aspirin triad") has been attributed to arachidonic metabolism alternations, although the putative biochemical defects have not been elucidated. The aim of this
study was assessment of the hypothesis that local production of eicosanoids in the respiratory epithelium in patients with ASA-sensitive asthma/rhinosinusitis (ASARS) differs from that of ASA-tolerant
patients with rhinosinusitis (ATRS). Nasal polyps were obtained from 10 patients with ASARS and 15 with ATRS during routine polypectomies, and epithelial cells (ECs) were cultured on bovine collagen
type I matrix (Vitrogen 100), in medium supplemented with growth factors. The generation of
eicosanoids in supernatants of confluent ECs (6 to 8 d of culture; purity > 98%) was quantified by immunoassays. Unstimulated ECs from ASARS patients generated significantly less prostaglandin E2(PGE2)
compared with ATRS (0.8 ± 0.3 versus 2.4 ± 0.5 ng/µg double-stranded deoxyribonucleic acid [dsDNA],
respectively), although a similar relative increase in response to calcium ionophore and inhibition
with ASA was observed in both groups. Basal levels of 15-hydroxyeicosatetraenoic acid (15-HETE)
were not different between groups, and calcium ionophore enhanced its production to a similar extent. However, cells incubation with 200 µM ASA for 60 min resulted in a significant increase (mean
+359%) in 15-HETE generation only in ASARS patients, whereas no effect of ASA on 15-HETE generation in ATRS patients was observed. PGF2 generation was similar in both groups, and no significant
generation of PGD2 or leukotriene C4 (LTC4) was observed in epithelial cell cultures from either
group. Our results indicate that nasal polyps ECs from ASA-sensitive patients have significant abnormality in basal and ASA-induced generation of eicosanoids which may be causally related to the mechanism of ASA sensitivity. Kowalski ML, Pawliczak R, Wozniak J, Siuda K, Poniatowska M, Iwaszkiewicz J, Kornatowski T, Kaliner MA. Differential metabolism of arachidonic acid in nasal
polyp epithelial cells cultured from aspirin-sensitive and aspirin-tolerant patients.
This article has been cited by other articles:

|
 |

|
 |
 
L. Pujols, P. Benitez, I. Alobid, A. Martinez-Anton, J. Roca-Ferrer, J. Mullol, and C. Picado
Glucocorticoid therapy increases COX-2 gene expression in nasal polyps in vivo
Eur. Respir. J.,
March 1, 2009;
33(3):
502 - 508.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Sakanaka, S. Tanaka, Y. Sugimoto, and A. Ichikawa
Essential role of EP3 subtype in prostaglandin E2-induced adhesion of mouse cultured and peritoneal mast cells to the Arg-Gly-Asp-enriched matrix
Am J Physiol Cell Physiol,
November 1, 2008;
295(5):
C1427 - C1433.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Farooque and T. H Lee
Aspirin sensitivity and eicosanoids
Thorax,
January 1, 2008;
63(1):
2 - 4.
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L Mastalerz, M Sanak, A Gawlewicz-Mroczka, A Gielicz, A Cmiel, and A Szczeklik
Prostaglandin E2 systemic production in patients with asthma with and without aspirin hypersensitivity
Thorax,
January 1, 2008;
63(1):
27 - 34.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J Roca-Ferrer, L Pujols, S Gartner, A Moreno, F Pumarola, J Mullol, N Cobos, and C Picado
Upregulation of COX-1 and COX-2 in nasal polyps in cystic fibrosis
Thorax,
July 1, 2006;
61(7):
592 - 596.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. H. Lee, M. V. Williams, R. N. DuBois, and I. A. Blair
Cyclooxygenase-2-mediated DNA Damage
J. Biol. Chem.,
August 5, 2005;
280(31):
28337 - 28346.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Antczak, P. Montuschi, S. Kharitonov, P. Gorski, and P. J. Barnes
Increased Exhaled Cysteinyl-Leukotrienes and 8-Isoprostane in Aspirin-induced Asthma
Am. J. Respir. Crit. Care Med.,
August 1, 2002;
166(3):
301 - 306.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. J. TOBIN
Asthma, Airway Biology, and Allergic Rhinitis in AJRCCM 2000
Am. J. Respir. Crit. Care Med.,
November 1, 2001;
164(9):
1559 - 1580.
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. L Kowalski
Rhinosinusitis and nasal polyposis in aspirin sensitive and aspirin tolerant patients: are they different?
Thorax,
October 1, 2000;
55(90002):
84S - 86.
[Full Text]
|
 |
|
Copyright © 2000 American Thoracic Society
|
|
|