help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by NAURECKAS, E. T.
Right arrow Articles by SOLWAY, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by NAURECKAS, E. T.
Right arrow Articles by SOLWAY, J.

Am. J. Respir. Crit. Care Med., Volume 160, Number 6, December 1999, 2062-2066

Bronchoalveolar Lavage Fluid from Asthmatic Subjects Is Mitogenic for Human Airway Smooth Muscle

EDWARD T. NAURECKAS, I. MAURICE NDUKWU, ANDREW J. HALAYKO, CHRISTOPHER MAXWELL, MARC B. HERSHENSON, and JULIAN SOLWAY

Section of Pulmonary and Critical Care Medicine and Department of Pediatrics, The University of Chicago, Chicago, Illinois

Airway smooth muscle proliferation may contribute to the airway wall remodeling seen in asthma. In this study we tested for the presence of airway smooth muscle mitogenic activity in bronchoalveolar lavage (BAL) fluid obtained from 12 atopic asthmatics before and serially after segmental allergen challenge, and from four normal subjects who did not undergo allergen challenge. Mitogenic effect was assessed by coincubating BAL fluid with human airway smooth muscle cells, and measuring its effect on 3[H]thymidine incorporation and cell number. Induction of ERK phosphorylation and cyclin D1 protein abundance were also assessed. Compared with serum-free medium alone, BAL fluid obtained from normal subjects increased thymidine incorporation, cell number, ERK phosphorylation, and cyclin D1 abundance. BAL fluid from asthmatic subjects prior to allergen challenge induced even greater increases in all measures, except for cell number, which was similar to that observed with normal subjects' BAL fluid. Incubation with lavage fluid obtained 48 h after segmental allergen challenge in atopic asthmatics caused yet further increases in thymidine incorporation, cell number, and cyclin D1 protein abundance. Molecular sieving of prechallenge BAL fluid from three asthmatic subjects demonstrated that mitogenic activity was present exclusively in the > 10 kD fraction. These results provide the first direct demonstration that fluid lining the airways of asthmatics contains excess mitogenic activity for human airway smooth muscle, and that this activity increases further after allergen challenge. Naureckas ET, Ndukwu IM, Halayko AJ, Maxwell C, Hershenson MB, Solway J. Bronchoalveolar lavage fluid from asthmatic subjects is mitogenic for human airway smooth muscle.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
D. Prefontaine, S. Lajoie-Kadoch, S. Foley, S. Audusseau, R. Olivenstein, A. J. Halayko, C. Lemiere, J. G. Martin, and Q. Hamid
Increased Expression of IL-33 in Severe Asthma: Evidence of Expression by Airway Smooth Muscle Cells
J. Immunol., October 15, 2009; 183(8): 5094 - 5103.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. E. Monzon, D. Manzanares, N. Schmid, S. M. Casalino-Matsuda, and R. M. Forteza
Hyaluronidase Expression and Activity Is Regulated by Pro-Inflammatory Cytokines in Human Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., September 1, 2008; 39(3): 289 - 295.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. C. Simeone-Penney, M. Severgnini, L. Rozo, S. Takahashi, B. H. Cochran, and A. R. Simon
PDGF-induced human airway smooth muscle cell proliferation requires STAT3 and the small GTPase Rac1
Am J Physiol Lung Cell Mol Physiol, April 1, 2008; 294(4): L698 - L704.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. A. Panettieri Jr., M. I. Kotlikoff, W. T. Gerthoffer, M. B. Hershenson, P. G. Woodruff, I. P. Hall, and S. Banks-Schlegel
Airway Smooth Muscle in Bronchial Tone, Inflammation, and Remodeling: Basic Knowledge to Clinical Relevance
Am. J. Respir. Crit. Care Med., February 1, 2008; 177(3): 248 - 252.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Joubert, S. Lajoie-Kadoch, M. Welman, S. Dragon, S. Letuvee, B. Tolloczko, A. J. Halayko, A. S. Gounni, K. Maghni, and Q. Hamid
Expression and Regulation of CCR1 by Airway Smooth Muscle Cells in Asthma
J. Immunol., January 15, 2008; 180(2): 1268 - 1275.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
J. K. Bentley and M. B. Hershenson
Airway Smooth Muscle Growth in Asthma: Proliferation, Hypertrophy, and Migration
Proceedings of the ATS, January 1, 2008; 5(1): 89 - 96.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. Sharma, T. Tran, G. L. Stelmack, K. McNeill, R. Gosens, M. M. Mutawe, H. Unruh, W. T. Gerthoffer, and A. J. Halayko
Expression of the dystrophin-glycoprotein complex is a marker for human airway smooth muscle phenotype maturation
Am J Physiol Lung Cell Mol Physiol, January 1, 2008; 294(1): L57 - L68.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. Zhang, L. Shan, M. S. Rahman, H. Unruh, A. J. Halayko, and A. S. Gounni
Constitutive and inducible thymic stromal lymphopoietin expression in human airway smooth muscle cells: role in chronic obstructive pulmonary disease
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L375 - L382.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. Gosens, G. Dueck, W. T. Gerthoffer, H. Unruh, J. Zaagsma, H. Meurs, and A. J. Halayko
p42/p44 MAP kinase activation is localized to caveolae-free membrane domains in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1163 - L1172.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
K. J. Greenlee, Z. Werb, and F. Kheradmand
Matrix Metalloproteinases in Lung: Multiple, Multifarious, and Multifaceted
Physiol Rev, January 1, 2007; 87(1): 69 - 98.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. Gosens, G. L. Stelmack, G. Dueck, K. D. McNeill, A. Yamasaki, W. T. Gerthoffer, H. Unruh, A. S. Gounni, J. Zaagsma, and A. J Halayko
Role of caveolin-1 in p42/p44 MAP kinase activation and proliferation of human airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L523 - L534.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. Borger, M. Tamm, J. L. Black, and M. Roth
Asthma: Is It Due to an Abnormal Airway Smooth Muscle Cell?
Am. J. Respir. Crit. Care Med., August 15, 2006; 174(4): 367 - 372.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Lajoie-Kadoch, P. Joubert, S. Letuve, A. J. Halayko, J. G. Martin, A. Soussi-Gounni, and Q. Hamid
TNF-{alpha} and IFN-{gamma} inversely modulate expression of the IL-17E receptor in airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1238 - L1246.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. S. Gounni, Q. Hamid, S. M. Rahman, J. Hoeck, J. Yang, and L. Shan
IL-9-Mediated Induction of Eotaxin1/CCL11 in Human Airway Smooth Muscle Cells
J. Immunol., August 15, 2004; 173(4): 2771 - 2779.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. Terada, E. A. B. Kelly, and N. N. Jarjour
Increased Thrombin Activity after Allergen Challenge: A Potential Link to Airway Remodeling?
Am. J. Respir. Crit. Care Med., February 1, 2004; 169(3): 373 - 377.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
A. M. Vignola, F. Mirabella, G. Costanzo, R. Di Giorgi, M. Gjomarkaj, V. Bellia, and G. Bonsignore
Airway Remodeling in Asthma
Chest, March 1, 2003; 123 (2009): 417S - 422S.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. Page, J. Li, K. C. Corbit, K. M. Rumilla, J.-W. Soh, I. B. Weinstein, C. Albanese, R. G. Pestell, M. R. Rosner, and M. B. Hershenson
Regulation of Airway Smooth Muscle Cyclin D1 Transcription by Protein Kinase C-{delta}
Am. J. Respir. Cell Mol. Biol., August 1, 2002; 27(2): 204 - 213.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. R. A. JOHNSON, M. ROTH, M. TAMM, M. HUGHES, Q. GE, G. KING, J. K. BURGESS, and J. L. BLACK
Airway Smooth Muscle Cell Proliferation Is Increased in Asthma
Am. J. Respir. Crit. Care Med., August 1, 2001; 164(3): 474 - 477.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. R. A. JOHNSON, J. L. BLACK, S. CARLIN, Q. GE, and P. ANNE UNDERWOOD
The Production of Extracellular Matrix Proteins by Human Passively Sensitized Airway Smooth-Muscle Cells in Culture . The Effect of Beclomethasone
Am. J. Respir. Crit. Care Med., December 1, 2000; 162(6): 2145 - 2151.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. F. Thomas Jr. and A. H. Limper
Phosphatidylinositol Kinase Regulation of Airway Smooth Muscle Cell Proliferation
Am. J. Respir. Cell Mol. Biol., October 1, 2000; 23(4): 429 - 430.
[Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. R. Simon, S. Takahashi, M. Severgnini, B. L. Fanburg, and B. H. Cochran
Role of the JAK-STAT pathway in PDGF-stimulated proliferation of human airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1296 - L1304.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1999 American Thoracic Society 		  New Orleans Int'l Conf