help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by FUJIEDA, S.
Right arrow Articles by SAITO, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by FUJIEDA, S.
Right arrow Articles by SAITO, H.

Am. J. Respir. Crit. Care Med., Volume 160, Number 6, December 1999, 2056-2061

DNA from Mycobacterium bovis Bacillus Calmette-Guérin (MY-1) Inhibits Immunoglobulin E Production by Human Lymphocytes

SHIGEHARU FUJIEDA, SUMIKO IHO, YUICHI KIMURA, HIROSHI SUNAGA, HIDEKI IGAWA, CHIZURU SUGIMOTO, SABURO YAMAMOTO, and HITOSHI SAITO

Departments of Otorhinolaryngology and Immunology, Fukui Medical University, Fukui; and Department of Bacterial and Blood Products, National Institute of Infectious Diseases, Tokyo, Japan

A DNA fraction purified from Mycobacterium bovis bacillus Calmette-Guérin (BCG) and designated MY-1 induced interferon (IFN)-gamma production by human peripheral blood mononuclear cells (PBMC). IFN-gamma is well known as a downregulator of IgE production. In this study we investigated whether MY-1 regulates IgE production by human PBMC in vitro. MY-1 inhibited IgE production in PBMC taken from normal donors and stimulated with interleukin (IL)-4 plus monoclonal anti-CD40 antibody, without affecting production of IgA. MY-1 enhanced production of IFN-gamma and IL-12 by PBMC. Inhibition by MY-1 of IgE production was mediated by both IFN-gamma and IL-12, since the MY-1-induced suppression was blocked by the addition of monoclonal anti-IFN-gamma antibody, monoclonal anti-IL-12 antibody or a monoclonal antibody (mAb) directed at the IL-12 receptor. MY-1 inhibited the induction of varepsilon  germ-line transcript by IL-4. Additionally, MY-1 inhibited spontaneous in vitro production of IgE by PBMC from atopic donors in the absence of IL-4 plus anti-CD40 mAb. These results suggest that exposure to MY-1 may be a novel strategy for the treatment of IgE-related allergic disease. Fujieda S, Iho S, Kimura Y, Sunaga H, Igawa H, Sugimoto C, Yamamoto S, Saito H. DNA from Mycobacterium bovis bacillus Calmette-Guérin (MY-1) inhibits immunoglobulin E production by human lymphocytes.




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
S. FUJIEDA, S. IHO, Y. KIMURA, H. YAMAMOTO, H. IGAWA, and H. SAITO
Synthetic Oligodeoxynucleotides Inhibit IgE Induction in Human Lymphocytes
Am. J. Respir. Crit. Care Med., July 1, 2000; 162(1): 232 - 239.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1999 American Thoracic Society 		  ATS Clinical Skills Tests