help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by ARMSTRONG, L.
Right arrow Articles by MILLAR, A. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ARMSTRONG, L.
Right arrow Articles by MILLAR, A. B.

Am. J. Respir. Crit. Care Med., Volume 160, Number 6, December 1999, 1910-1915

Changes in Collagen Turnover in Early Acute Respiratory Distress Syndrome

LYNNE ARMSTRONG, DAVID R. THICKETT, JASON P. MANSELL, MIRELA IONESCU, EMMA HOYLE, R. CLARK BILLINGHURST, A. ROBIN POOLE, and ANN B. MILLAR

Lung Research Group, University of Bristol Department of Hospital Medicine, Division of Medicine, and Developmental Biology, University of Bristol Department of Child Dental Health, Bristol, United Kingdom; Joint Diseases Laboratory, Shriner's Hospitals for Children, Division of Surgical Research, Department of Surgery, McGill University, Montreal, Quebec, Canada; and Department of Clinical Sciences, Colorado State University, Fort Collins, Colorado

Pulmonary fibrosis is a well-recognized feature of acute respiratory distress syndrome (ARDS). Using immunoassays of bronchoalveolar lavage (BAL), fluid we investigated the synthesis of type I procollagen (PICP) and type I/II collagen degradation products (COL2-3/4Cshort neoepitope) in patients with ARDS, acute lung injury (ALI), subjects with risk factors for ARDS (At Risk), and healthy/ventilated control subjects. PICP was measured by ELISA as a marker of type I procollagen synthesis. COL2-3/4Cshort neoepitope was measured by an inhibition ELISA as a marker of collagenase degradation of type I/II collagen. BAL was performed initially within 48 h of ventilation (Day 1) and then subsequently on Day 4. Dilution of epithelial lining fluid (ELF) was corrected for by plasma urea comparison. Increased PICP levels were observed in the ELF from ARDS and ALI subjects on Day 1 compared with subjects At Risk (median values, 124.9 and 95.0 ng/ml versus 38.0 ng/ml, respectively, p < 0.0005). By contrast, the levels of COL2-3/4Cshort neoepitope were significantly reduced in the subjects with ARDS versus the At Risk subjects (13.22 ng/ml versus 32.33 ng/ml, p < 0.0005). This translated into a greatly increased PICP:COL2-3/4Cshort ratio in the subjects with ARDS (p < 0.0001). There was a significant decline in the PICP level in the subjects with ARDS between Days 1 and 4 (n = 15, p < 0.05). Linear regression analysis showed a significant association between PICP and lung injury score in the subjects with ARDS (p = 0.01). Our data suggests an early shift in balance between type I collagen synthesis and degradation by collagenase. The resultant increase in type I collagen would favor matrix deposition and the development of pulmonary fibrosis in the lungs of subjects with ARDS. Armstrong L, Thickett DR, Mansell JP, Ionescu M, Hoyle E, Billinghurst RC, Poole AR, Millar AB. Changes in collagen turnover in early acute respiratory distress syndrome.




This article has been cited by other articles:


Home page
 ThoraxHome page
P T G Elkington and J S Friedland
Matrix metalloproteinases in destructive pulmonary pathology
Thorax, March 1, 2006; 61(3): 259 - 266.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. L. Farias, D. S. Faffe, D. G. Xisto, M. C. E. Santana, R. Lassance, L. F. M. Prota, M. B. Amato, M. M. Morales, W. A. Zin, and P. R. M. Rocco
Positive end-expiratory pressure prevents lung mechanical stress caused by recruitment/derecruitment
J Appl Physiol, January 1, 2005; 98(1): 53 - 61.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. R. M. Rocco, A. B. Souza, D. S. Faffe, C. P. Passaro, F. B. Santos, E. M. Negri, J. G. M. Lima, R. S. Contador, V. L. Capelozzi, and W. A. Zin
Effect of Corticosteroid on Lung Parenchyma Remodeling at an Early Phase of Acute Lung Injury
Am. J. Respir. Crit. Care Med., September 15, 2003; 168(6): 677 - 684.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
P. Pelosi, D. D'Onofrio, D. Chiumello, S. Paolo, G. Chiara, V.L. Capelozzi, C.S.V. Barbas, M. Chiaranda, and L. Gattinoni
Pulmonary and extrapulmonary acute respiratory distress syndrome are different
Eur. Respir. J., August 1, 2003; 22(42_suppl): 48s - 56s.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. J. Fahy, F. Lichtenberger, C. B. McKeegan, G. J. Nuovo, C. B. Marsh, and M. D. Wewers
The Acute Respiratory Distress Syndrome: A Role for Transforming Growth Factor-{beta}1
Am. J. Respir. Cell Mol. Biol., April 1, 2003; 28(4): 499 - 503.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. K. Winkler and J. L. Fowlkes
Metalloproteinase and growth factor interactions: do they play a role in pulmonary fibrosis?
Am J Physiol Lung Cell Mol Physiol, July 1, 2002; 283(1): L1 - L11.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1999 American Thoracic Society 		  ATS Clinical Skills Tests