Am. J. Respir. Crit. Care Med.,
Volume 160, Number 5, November 1999, 1743-1749
Protective Effect of 3-Aminobenzamide, an Inhibitor
of Poly (ADP-Ribose) Synthetase, against Laryngeal
Injury in Rats
YORAM
STERN,
ANDREW
SALZMAN,
ROBIN T.
COTTON,
and
BASILIA
ZINGARELLI
Department of Otolaryngology and Division of Critical Care Medicine, Children's Hospital Medical Center, Cincinnati, Ohio
The effect of 3-aminobenzamide, an inhibitor of poly (ADP-ribose) synthetase activity, was evaluated
in a rat model of laryngeal injury induced by endotracheal intubation for 1 h. At 1 h after extubation,
the laryngeal damage was characterized by areas of mucosal necrosis, submucosal edema, swelling
of subglottic glands, and submucosal infiltration of inflammatory cells. Activity of myeloperoxidase, a
marker of neutrophil infiltration, was also markedly increased into the damaged tissue. Immunohistochemistry for nitrotyrosine, an index of nitrosative stress, showed an intense staining in the inflamed larynx. Treatment with 3-aminobenzamide (10 mg/kg intraperitoneally) significantly reduced the appearance of mucosal damage and was associated with a significant reduction of tissue
myeloperoxidase activity and nitrotyrosine immunoreactivity in the larynx. The results of this study
suggest that poly (ADP-ribose) synthetase may play a role in the inflammatory process after laryngeal intubation and extubation, and administration of 3-aminobenzamide may be a beneficial therapeutic approach. Stern Y, Salzman A, Cotton RT, Zingarelli B. Protective effect of 3-aminobenzamide, an inhibitor of poly (ADP-ribose) synthetase, against laryngeal injury in rats.
