help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by OCHS, M.
Right arrow Articles by FEHRENBACH, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by OCHS, M.
Right arrow Articles by FEHRENBACH, H.

Am. J. Respir. Crit. Care Med., Volume 160, Number 2, August 1999, 718-724

Ultrastructural Alterations in Intraalveolar Surfactant Subtypes after Experimental Ischemia and Reperfusion

MATTHIAS OCHS, ILIJA NENADIC, ANTONIA FEHRENBACH, JOHANNES M. ALBES, THORSTEN WAHLERS, JOACHIM RICHTER, and HEINZ FEHRENBACH

Division of Electron Microscopy, Department of Anatomy, University of Göttingen, Göttingen, Germany; Division of Thoracic and Cardiovascular Surgery, Medical School Hannover, Hannover, Germany; and Institute of Pathology, University Clinics Carl Gustav Carus, TU Dresden, Dresden, Germany

Ischemia and reperfusion (I/R) result in surfactant dysfunction. Whether the impairment of surfactant is a consequence or a cause of intraalveolar edema formation is still unknown. The cumulative effects of lung perfusion, ischemic storage, and subsequent reperfusion on surfactant ultrastructure and pulmonary function were studied in a rat isolated perfused lung model. The left lungs were fixed for electron microscopy by vascular perfusion either immediately after excision (control; n = 5) or after perfusion with modified Euro-Collins solution (EC), storage for 2 h at 4° C in EC, and reperfusion for 40 min (n = 5). A stereological approach was chosen to discriminate between intraalveolar surfactant subtypes of edematous regions and regions free of edema. Intraalveolar edema seen after I/R in the EC group occupied 36 ± 6% (mean ± SEM) of the gas exchange region as compared with control lungs (1 ± 1%; p = 0.008). Relative intraalveolar surfactant composition showed a decrease in surface active tubular myelin (3 ± 1 versus 12 ± 0%; p = 0.008) and an increase in inactive unilamellar forms (83 ± 2 versus 64 ± 5%; p = 0.008) in the EC group. These changes occurred both in edematous (tubular myelin, 3 ± 1%; unilamellar forms, 88 ± 6%) and in nonedematous regions (tubular myelin, 4 ± 3%; unilamellar forms, 77 ± 5%). The ultrastructural changes in surfactant were associated with an increase in peak inspiratory pressure during reperfusion. In conclusion, surfactant alterations seen after I/R are not directly related to the presence of edema fluid in the alveoli. Disturbances in intraalveolar surfactant after I/R are not merely the result of inactivation due to plasma protein leakage but may instead be responsible for an increased permeability of the blood-air barrier, resulting in a vicious cycle of intraalveolar edema formation and progressing surfactant impairment.




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
E. R. Weibel, C. C. W. Hsia, and M. Ochs
How much is there really? Why stereology is essential in lung morphometry
J Appl Physiol, January 1, 2007; 102(1): 459 - 467.
[Abstract] [Full Text] [PDF]

Home page
 ERRHome page
M. Ochs
Stereological analysis of acute lung injury
Eur. Respir. Rev., December 1, 2006; 15(101): 115 - 121.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
A. Gunther, M. Balser, R. Schmidt, P. Markart, A. Olk, J. Borgermann, F. H. Splittgerber, W. Seeger, and I. Friedrich
Surfactant abnormalities after single lung transplantation in dogs: Impact of bronchoscopic surfactant administration
J. Thorac. Cardiovasc. Surg., February 1, 2004; 127(2): 344 - 354.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. de Perrot, M. Liu, T. K. Waddell, and S. Keshavjee
Ischemia-Reperfusion-induced Lung Injury
Am. J. Respir. Crit. Care Med., February 15, 2003; 167(4): 490 - 511.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Ochs, G. Johnen, K.-M. Muller, T. Wahlers, S. Hawgood, J. Richter, and F. Brasch
Intracellular and Intraalveolar Localization of Surfactant Protein A (SP-A) in the Parenchymal Region of the Human Lung
Am. J. Respir. Cell Mol. Biol., January 1, 2002; 26(1): 91 - 98.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. FEHRENBACH, M. OCHS, T. WARNECKE, T. WAHLERS, T. WITTWER, A. SCHMIEDL, S. ELKI, D. MEYER, J. RICHTER, and H. FEHRENBACH
Beneficial Effect of Lung Preservation Is Related to Ultrastructural Integrity of Tubular Myelin after Experimental Ischemia and Reperfusion
Am. J. Respir. Crit. Care Med., June 1, 2000; 161(6): 2058 - 2065.
[Abstract] [Full Text]

Home page
 RadiologyHome page
E. M. Marom, Y. W. Choi, S. M. Palmer, D. M. DeLong, M. D. Stuart, and H. P. McAdams
Reperfusion Edema after Lung Transplantation: Effect of Daclizumab
Radiology, November 1, 2001; 221(2): 508 - 514.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1999 American Thoracic Society