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Am. J. Respir. Crit. Care Med., Volume 159, Number 6, June 1999, 1981-1984

Analysis of the Kveim-Siltzbach Test Reagent for Bacterial DNA

ELVIRA RICHTER, Y. P. KATARIA, G. ZISSEL, J. HOMOLKA, M. SCHLAAK, and J. MÜLLER-QUERNHEIM

Research Centre Borstel, Medical Hospital, and National Reference Center for Mycobacteria, Borstel, Germany; Pulmonary and Critical Care Section, Department of Medicine, East Carolina University School of Medicine, Greenville, North Carolina; and First Lung Department, Charles University, Prague, Czech Republic

The sarcoid spleen-derived reagent for the Kveim-Siltzbach test (KST) elicits a sarcoid-specific, granulomatous, cutaneous response used to establish the diagnosis of sarcoidosis. In the context of the ongoing discussion of a bacterial cause of sarcoidosis we asked the question whether bacterial DNA could be found in the KST reagent. For this purpose two different KST reagents, an identical preparation from a normal spleen, and a native sarcoid spleen were analyzed by polymerase chain reaction (PCR) employing universal primers detecting conserved DNA sequences coding for bacterial ribosomal 16S RNA. Neither KST reagents, the control preparation, nor the spleen yielded a positive signal, indicating that the preparations are free of bacterial contamination. Because the KST reagent elicits granuloma, these results do not support the hypothesis of a bacterial cause of sarcoid granuloma.




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