help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by HASHIMOTO, S.
Right arrow Articles by HORIE, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by HASHIMOTO, S.
Right arrow Articles by HORIE, T.

Am. J. Respir. Crit. Care Med., Volume 159, Number 2, February 1999, 634-640

Hyperosmolarity-induced Interleukin-8 Expression in Human Bronchial Epithelial Cells through p38 Mitogen-activated Protein Kinase

SHU HASHIMOTO, KEN MATSUMOTO, YASUHIRO GON, TOMOKO NAKAYAMA, IKUKO TAKESHITA, and TAKASHI HORIE

First Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan

The changes in airway osmolarity have been described to contribute to the production of exercise- induced bronchoconstriction (EIB) and the development of the late-phase response (LPR). The mechanism has been investigated; however, the responsiveness of bronchial epithelial cells (BEC) to hyperosmolarity and the intracellular signals leading to cell activation have not been determined. In this study, we examined the effect of hyperosmolar medium on interleukin-8 (IL-8) expression and the role of p38 mitogen-activated protein (MAP) kinase and c-Jun NH2 terminal kinase ( JNK) in human BEC in this response in order to clarify the intracellular signals regulating IL-8 expression in hyperosmolarity-stimulated BEC. The results showed that hyperosmolarity induced IL-8 expression in a concentration dependent manner, p38 MAP kinase phosphorylation and activation, and JNK activation whether NaCl or mannitol was used as the solute. SB 203580 as the specific p38 MAP kinase inhibitor inhibited hyperosmolarity-induced p38 MAP kinase activation and partially inhibited hyperosmolarity-induced IL-8 expression. These results indicate that p38 MAP kinase, at least in part, regulates hyperosmolarity-induced IL-8 expression in BEC. However, other signals such as JNK are possibly also involved. These results provide new evidence on the mechanism responsible for the development of the LPR induced by EIB, and a strategy for treatment with the specific p38 MAP kinase inhibitor.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
K. P. Y. Hui, S. M. Y. Lee, C.-y. Cheung, I. H. Y. Ng, L. L. M. Poon, Y. Guan, N. Y. Y. Ip, A. S. Y. Lau, and J. S. M. Peiris
Induction of Proinflammatory Cytokines in Primary Human Macrophages by Influenza A Virus (H5N1) Is Selectively Regulated by IFN Regulatory Factor 3 and p38 MAPK
J. Immunol., January 15, 2009; 182(2): 1088 - 1098.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Schwartz, M. Abolhassani, M. Pooya, J.-M. Steyaert, X. Wertz, M. Israel, A. Guais, and P. Chaumet-Riffaud
Hyperosmotic stress contributes to mouse colonic inflammation through the methylation of protein phosphatase 2A
Am J Physiol Gastrointest Liver Physiol, November 1, 2008; 295(5): G934 - G941.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
Y. Jing, J. A. Dowdy, M. R. Van Scott, and J. S. Fedan
Hyperosmolarity-Induced Dilation and Epithelial Bioelectric Responses of Guinea Pig Trachea in Vitro: Role of Kinase Signaling
J. Pharmacol. Exp. Ther., July 1, 2008; 326(1): 186 - 195.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
L. Chimenti, G. Morici, A. Paterno, A. Bonanno, L. Siena, A. Licciardi, M. Veca, W. Guccione, F. Macaluso, G. Bonsignore, et al.
Endurance Training Damages Small Airway Epithelium in Mice
Am. J. Respir. Crit. Care Med., March 1, 2007; 175(5): 442 - 449.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
H. van der Vaart, D. S. Postma, W. Timens, H. F. Kauffman, M. N. Hylkema, and N. H.T. ten Hacken
Repeated sputum inductions induce a transient neutrophilic and eosinophilic response.
Chest, October 1, 2006; 130(4): 1157 - 1164.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y.-M. Kim, W. Reed, W. Wu, P. A. Bromberg, L. M. Graves, and J. M. Samet
Zn2+-induced IL-8 expression involves AP-1, JNK, and ERK activities in human airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2006; 290(5): L1028 - L1035.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
G. E. Carpagnano, M P. Foschino Barbaro, M. Cagnazzo, G Di Gioia, T Giliberti, C Di Matteo, and O Resta
Use of Exhaled Breath Condensate in the Study of Airway Inflammation After Hypertonic Saline Solution Challenge
Chest, November 1, 2005; 128(5): 3159 - 3166.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
T. S. Hallstrand, M. W. Moody, M. M. Wurfel, L. B. Schwartz, W. R. Henderson Jr., and M. L. Aitken
Inflammatory Basis of Exercise-induced Bronchoconstriction
Am. J. Respir. Crit. Care Med., September 15, 2005; 172(6): 679 - 686.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. S. Davis, J. R. Malayer, L. Vandeventer, C. M. Royer, E. C. McKenzie, and K. K. Williamson
Cold weather exercise and airway cytokine expression
J Appl Physiol, June 1, 2005; 98(6): 2132 - 2136.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Roux, H. Kawakatsu, B. Gartland, M. Pespeni, D. Sheppard, M. A. Matthay, C. M. Canessa, and J.-F. Pittet
Interleukin-1{beta} Decreases Expression of the Epithelial Sodium Channel {alpha}-Subunit in Alveolar Epithelial Cells via a p38 MAPK-dependent Signaling Pathway
J. Biol. Chem., May 13, 2005; 280(19): 18579 - 18589.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y.-M. Kim, W. Reed, A. G. Lenz, I. Jaspers, R. Silbajoris, H. S. Nick, and J. M. Samet
Ultrafine carbon particles induce interleukin-8 gene transcription and p38 MAPK activation in normal human bronchial epithelial cells
Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L432 - L441.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
D.-Q. Li, Z. Chen, X. J. Song, L. Luo, and S. C. Pflugfelder
Stimulation of Matrix Metalloproteinases by Hyperosmolarity via a JNK Pathway in Human Corneal Epithelial Cells
Invest. Ophthalmol. Vis. Sci., December 1, 2004; 45(12): 4302 - 4311.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. L. Booth, K. M. Coggeshall, B. E. Gordon, and J. P. Metcalf
Adenovirus Type 7 Induces Interleukin-8 in a Lung Slice Model and Requires Activation of Erk
J. Virol., April 15, 2004; 78(8): 4156 - 4164.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Frank, J. Roux, H. Kawakatsu, G. Su, A. Dagenais, Y. Berthiaume, M. Howard, C. M. Canessa, X. Fang, D. Sheppard, et al.
Transforming Growth Factor-{beta}1 Decreases Expression of the Epithelial Sodium Channel {alpha}ENaC and Alveolar Epithelial Vectorial Sodium and Fluid Transport via an ERK1/2-dependent Mechanism
J. Biol. Chem., November 7, 2003; 278(45): 43939 - 43950.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
O. Tabary, C. Muselet, S. Escotte, F. Antonicelli, D. Hubert, D. Dusser, and J. Jacquot
Interleukin-10 Inhibits Elevated Chemokine Interleukin-8 and Regulated on Activation Normal T Cell Expressed and Secreted Production in Cystic Fibrosis Bronchial Epithelial Cells by Targeting the IkB Kinase {alpha}/{beta} Complex
Am. J. Pathol., January 1, 2003; 162(1): 293 - 302.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Li, S. Kartha, S. Iasvovskaia, A. Tan, R. K. Bhat, J. M. Manaligod, K. Page, A. R. Brasier, and M. B. Hershenson
Regulation of human airway epithelial cell IL-8 expression by MAP kinases
Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L690 - L699.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. S. Davis, B. McKiernan, S. McCullough, S. Nelson Jr., R. E. Mandsager, M. Willard, and K. Dorsey
Racing Alaskan Sled Dogs as a Model of "Ski Asthma"
Am. J. Respir. Crit. Care Med., September 15, 2002; 166(6): 878 - 882.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
Z. H. Nemeth, E. A. Deitch, C. Szabo, and G. Hasko
Hyperosmotic Stress Induces Nuclear Factor-{kappa}B Activation and Interleukin-8 Production in Human Intestinal Epithelial Cells
Am. J. Pathol., September 1, 2002; 161(3): 987 - 996.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Cummings, N. L. Parinandi, A. Zaiman, L. Wang, P. V. Usatyuk, J. G. N. Garcia, and V. Natarajan
Phospholipase D Activation by Sphingosine 1-Phosphate Regulates Interleukin-8 Secretion in Human Bronchial Epithelial Cells
J. Biol. Chem., August 9, 2002; 277(33): 30227 - 30235.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. Suri, L. J. Marshall, C. Wallis, C. Metcalfe, A. Bush, and J. K. Shute
Effects of Recombinant Human DNase and Hypertonic Saline on Airway Inflammation in Children with Cystic Fibrosis
Am. J. Respir. Crit. Care Med., August 1, 2002; 166(3): 352 - 355.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Oudin and J. Pugin
Role of MAP Kinase Activation in Interleukin-8 Production by Human BEAS-2B Bronchial Epithelial Cells Submitted to Cyclic Stretch
Am. J. Respir. Cell Mol. Biol., July 1, 2002; 27(1): 107 - 114.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. S. Kerby, V. Cottin, F. J. Accurso, F. Hoffmann, E. D. Chan, V. A. Fadok, and D. W. H. Riches
Impairment of macrophage survival by NaCl: implications for early pulmonary inflammation in cystic fibrosis
Am J Physiol Lung Cell Mol Physiol, July 1, 2002; 283(1): L188 - L197.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. TAUBE, O. HOLZ, M. MUCKE, R. A. JORRES, and H. MAGNUSSEN
Airway Response to Inhaled Hypertonic Saline in Patients with Moderate to Severe Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med., November 15, 2001; 164(10): 1810 - 1815.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. J. TSCHUMPERLIN and J. M. DRAZEN
Mechanical Stimuli to Airway Remodeling
Am. J. Respir. Crit. Care Med., November 15, 2001; 164(10): S90 - 94.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. S. DAVIS and A. N. FREED
Repeated Hyperventilation Causes Peripheral Airways Inflammation, Hyperreactivity, and Impaired Bronchodilation in Dogs
Am. J. Respir. Crit. Care Med., September 1, 2001; 164(5): 785 - 789.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Kawasaki, H. Takizawa, K. Takami, M. Desaki, H. Okazaki, T. Kasama, K. Kobayashi, K. Yamamoto, K. Nakahara, M. Tanaka, et al.
Benzene-Extracted Components Are Important for the Major Activity of Diesel Exhaust Particles . Effect on Interleukin-8 Gene Expression in Human Bronchial Epithelial Cells
Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 419 - 426.
[Abstract] [Full Text]

Home page
 ThoraxHome page
N R Henig, M R Tonelli, M V Pier, J L Burns, and M L Aitken
Sputum induction as a research tool for sampling the airways of subjects with cystic fibrosis
Thorax, April 1, 2001; 56(4): 306 - 311.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. HASHIMOTO, Y. GON, I. TAKESHITA, K. MATSUMOTO, S. MARUOKA, and T. HORIE
Transforming Growth Factor-{beta}1 Induces Phenotypic Modulation of Human Lung Fibroblasts to Myofibroblast Through a c-Jun-NH2-Terminal Kinase-Dependent Pathway
Am. J. Respir. Crit. Care Med., January 1, 2001; 163(1): 152 - 157.
[Abstract] [Full Text]

Home page
 J. Appl. Physiol.Home page
R. Suzuki and A. N. Freed
Hypertonic saline aerosol increases airway reactivity in the canine lung periphery
J Appl Physiol, December 1, 2000; 89(6): 2139 - 2146.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. C. Underwood, R. R. Osborn, S. Bochnowicz, E. F. Webb, D. J. Rieman, J. C. Lee, A. M. Romanic, J. L. Adams, D. W. P. Hay, and D. E. Griswold
SB 239063, a p38 MAPK inhibitor, reduces neutrophilia, inflammatory cytokines, MMP-9, and fibrosis in lung
Am J Physiol Lung Cell Mol Physiol, November 1, 2000; 279(5): L895 - L902.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. D. Griego, C. B. Weston, J. L. Adams, R. Tal-Singer, and S. B. Dillon
Role of p38 Mitogen-Activated Protein Kinase in Rhinovirus-Induced Cytokine Production by Bronchial Epithelial Cells
J. Immunol., November 1, 2000; 165(9): 5211 - 5220.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. HASHIMOTO, Y. GON, K. MATSUMOTO, I. TAKESHITA, S. MARUOKA, and T. HORIE
Inhalant Corticosteroids Inhibit Hyperosmolarity-induced, and Cooling and Rewarming-induced Interleukin-8 and RANTES Production by Human Bronchial Epithelial Cells
Am. J. Respir. Crit. Care Med., September 1, 2000; 162(3): 1075 - 1080.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. Hashimoto, Y. Gon, K. Matsumoto, S. Maruoka, I. Takeshita, S. Hayashi, Y. Asai, Y. Asai, I. Jibiki, T. Machino, et al.
Selective Inhibitor of p38 Mitogen-Activated Protein Kinase Inhibits Lipopolysaccharide-Induced Interleukin-8 Expression in Human Pulmonary Vascular Endothelial Cells
J. Pharmacol. Exp. Ther., May 1, 2000; 293(2): 370 - 375.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. MARUOKA, S. HASHIMOTO, Y. GON, I. TAKESHITA, and T. HORIE
PAF-induced RANTES Production by Human Airway Smooth Muscle Cells Requires Both p38 MAP Kinase and Erk
Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): 922 - 929.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. HASHIMOTO, Y. GON, I. TAKESHITA, K. MATSUMOTO, I. JIBIKI, H. TAKIZAWA, S. KUDOH, and T. HORIE
Diesel Exhaust Particles Activate p38 MAP Kinase to Produce Interleukin 8 and RANTES by Human Bronchial Epithelial Cells and N-Acetylcysteine Attenuates p38 MAP Kinase Activation
Am. J. Respir. Crit. Care Med., January 1, 2000; 161(1): 280 - 285.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1999 American Thoracic Society 		  CCM abstracts