help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by WESSELIUS, L. J.
Right arrow Articles by WIEGMANN, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by WESSELIUS, L. J.
Right arrow Articles by WIEGMANN, T.

Am. J. Respir. Crit. Care Med., Volume 159, Number 1, January 1999, 100-106

Iron Uptake Promotes Hyperoxic Injury to Alveolar Macrophages

LEWIS J. WESSELIUS, WADE L. WILLIAMS, KIRSTIN BAILEY, SUSAN VAMOS, AMY R. O'BRIEN-LADNER, and THOMAS WIEGMANN

Department of Medicine, Kansas City Veterans Affairs Medical Center, Kansas City, Missouri; and Department of Medicine, University of Kansas School of Medicine, Kansas City, Kansas

Iron uptake by cells may increase the intracellular pool of prooxidant iron prior to storage of iron within ferritin. Because hyperoxia is toxic to alveolar macrophages (AM) via mechanisms involving oxidant stress, we hypothesized that iron uptake by AM might promote hyperoxia-induced injury. To assess this hypothesis, we cultured AM recovered from healthy volunteers under conditions of normoxia or hyperoxia (60% or 95% oxygen) in media of varying iron content, including control media (3 µM iron) and media supplemented with iron (FeCl3; total iron 10, 20, or 40 µM). AM injury was assessed by measuring release of lactate dehydrogenase (LDH), phagocytic activity for yeast, and cytosolic concentrations of calcium ([Ca2+]i) as determined by ratio image analysis of AM loaded with the fluorescent calcium probe indo-1. There was dose-dependent accumulation of iron and ferritin synthesis in AM exposed to iron-supplemented media. Exposure of AM to hyperoxia (60% and 95% oxygen, 18 h) in control media increased LDH release and impaired phagocytic activity for yeast; however, similar hyperoxic exposures in iron-supplemented media significantly increased the cells' LDH release and decreased phagocytosis. Exposure to 95% oxygen increased the [Ca2+]i of AM over 18 h, but similar exposure in iron-supplemented media induced greater increases in [Ca2+]i. As compared with exposure to normoxia, exposure to hyperoxia (60% and 95% oxygen) also decreased iron uptake and, to a greater extent, ferritin synthesis by AM in iron-supplemented media. These data suggest that: (1) iron uptake promotes hyperoxic injury to AM; and (2) hyperoxia impairs the capacity of AM to sequester iron in ferritin.




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
J. M. Beck, A. M. Preston, S. E. Wilcoxen, S. B. Morris, E. S. White, and R. Paine III
Pneumocystis Pneumonia Increases the Susceptibility of Mice to Sublethal Hyperoxia
Infect. Immun., October 1, 2003; 71(10): 5970 - 5978.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
R.L. Upton, Y. Chen, S. Mumby, J.M.C. Gutteridge, P.B. Anning, A.G. Nicholson, T.W. Evans, and G.J. Quinlan
Variable tissue expression of transferrin receptors: relevance to acute respiratory distress syndrome
Eur. Respir. J., August 1, 2003; 22(2): 335 - 341.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
F. Yang, X. Wang, D. J. Haile, C. A. Piantadosi, and A. J. Ghio
Iron increases expression of iron-export protein MTP1 in lung cells
Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L932 - L939.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1999 American Thoracic Society 		  CCM abstracts