help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by SILVA, M. F. R.
Right arrow Articles by SALDIVA, P. H. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SILVA, M. F. R.
Right arrow Articles by SALDIVA, P. H. N.

Am. J. Respir. Crit. Care Med., Volume 158, Number 4, October 1998, 1230-1234

Airspace Configuration at Different Transpulmonary Pressures in Normal and Paraquat-induced Lung Injury in Rats

MARJORIE F. R. SILVA, WALTER A. ZIN, and PAULO H. N. SALDIVA

Laboratório de Poluição Atmosférica Experimental, Faculdade de Medicina da Universidade de São Paulo, São Paulo; and Instituto de Biofísica Carlos Chagas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

The aim of this study is to evaluate histoarchitecture of airspaces at different positive transpulmonary pressures, both during inflation and deflation of excised normal and paraquat-damaged rat lungs. Freshly excised lungs were placed in a plethysmograph connected to a graded pipette. Immediately after the achievement of the desired pressure level (5, 15, 25 cm H2O during inflation, and 15 and 5 cm H2O during deflation), the tracheal cannula was occluded and lungs were quick-frozen by immersion in liquid nitrogen, and fixed using Carnoy's solution. Sections representing the central and peripheral areas of the lungs were sampled and processed for paraffin embedding and stained with hematoxylin-eosin. By means of stereological techniques, surface-to-volume ratio (S/V) and the fraction of large-volume gas-exchanging airspaces (LVGEAS) were determined. We observed in paraquat-treated animals evidence of alveolar instability close to the resting volume. Structural unevenness was minimized by massive recruitment followed by alveolar pressurization. In conclusion, this work demonstrates that morphological evidence of uneven distribution of inspired air may be partially reversed by applying larger alveolar pressures. These larger pressures applied at the end-expiration in vivo (positive end-expiratory pressure, PEEP) can minimize the distortion of lung microarchitecture during ventilation.




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
T. Mauad, D. H. R. F. Rivero, R. C. de Oliveira, A. J. de Faria Coimbra Lichtenfels, E. T. Guimaraes, P. A. de Andre, D. I. Kasahara, H. M. de Siqueira Bueno, and P. H. N. Saldiva
Chronic Exposure to Ambient Levels of Urban Particles Affects Mouse Lung Development
Am. J. Respir. Crit. Care Med., October 1, 2008; 178(7): 721 - 728.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. A. Thomson, B. A. Yoder, V. T. Winter, H. Martin, D. Catland, T. M. Siler-Khodr, and J. J. Coalson
Treatment of Immature Baboons for 28 Days with Early Nasal Continuous Positive Airway Pressure
Am. J. Respir. Crit. Care Med., May 1, 2004; 169(9): 1054 - 1062.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1998 American Thoracic Society 		  ATS Coding and Billing Quarterly