Am. J. Respir. Crit. Care Med., Volume 158, Number 4, October 1998, 1204-1212

Filtration of Diaspirin Crosslinked Hemoglobin into Lung and Soft Tissue Lymph

ROBERT L. CONHAIM, STEPHENIA D. COOLER, ANN M. MCGRATH, DAVID A. DEANGELES, GREGORY A. MYERS, and BRUCE A. HARMS

Department of Surgery, University of Wisconsin-Madison, and William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin

Diaspirin crosslinked hemoglobin (DCHb) is a new blood substitute manufactured from human blood. To evaluate its microvascular filtration properties, we infused DCLHb into unanesthetized sheep (10%, 20 ml/kg) and measured the flow and composition of lung and soft tissue lymph. For comparison, we also infused human serum albumin (HSA; 10%, 20 ml/kg). DCLHb raised systemic and pulmonary arterial pressures from baseline values of 83 ± 7 and 13 ± 2 mm Hg, respectively, to peak values of 113 ± 9 and 26 ± 3 mm Hg (p < 0.05 versus baseline). These increases were significantly greater than those associated with HSA, which raised systemic and pulmonary arterial pressures from baseline values of 86 ± 4 and 13 ± 2 mm Hg, respectively, to peak values of 97 ± 3 and 21 ± 7 mm Hg (p  0.05 versus baseline and versus DCLHb). These differences reflect the known pressor properties of DCLHb. Accordingly, DCLHb raised lung and soft tissue lymph flows to peak values of 12.2 ± 3.8 and 1.6 ± 0.7 ml/30 min, respectively, while HSA raised lung and soft tissue lymph flows to peak values of 7.5 ± 4.8 and 4.6 ± 1.9 ml/30 min, respectively (p  0.05 versus DCLHb). The half-times of DCLHb equilibration from plasma into lung and soft tissue lymph of 1.0 ± 0.3 and 2.1 ± 1.1 h, respectively, were significantly faster than HSA equilibration half-times of 3.1 ± 0.2 and 3.8 ± 0.9 h. Filtration differences between DCLHb and HSA appear to be due to the pressor properties DCLHb.

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