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Am. J. Respir. Crit. Care Med., Volume 158, Number 1, July 1998, 241-248

Effects of Rhinovirus Infection on Hydrogen Peroxide- induced Alterations of Barrier Function in the Cultured Human Tracheal Epithelium

TAKASHI OHRUI, MUTSUO YAMAYA, KIYOHISA SEKIZAWA, NORIHIRO YAMADA, TOMOKO SUZUKI, MASANORI TERAJIMA, SHOJI OKINAGA, and HIDETADA SASAKI

Department of Geriatric Medicine, Tohoku University School of Medicine, Sendai 980, Japan

To investigate whether rhinovirus infection impairs epithelial barrier functions, human rhinovirus 14 (HRV-14) was infected to primary cultures of human tracheal epithelial cells and experiments were performed on Day 2 after HRV-14 infection. Hydrogen peroxide (H2O2; 3 × 10-4 M) increased electrical conductance (G) across the epithelial cell sheet measured with Ussing's chamber methods. Exposure of the epithelial cells to HRV-14 had no effect on H2O2-induced increases in G and [3H]mannitol flux through the cultured epithelium in the control condition, but it markedly potentiated H2O2- induced increases in both parameters in IL-1beta (100 U/ml) pretreated condition. However, pretreatment with TNF-alpha (100 U/ml) was without effect. IL-1beta enhanced the intercellular adhesion molecule-1 (ICAM-1) expression assessed by immunohistochemical analysis and susceptibility of epithelial cells to HRV-14 infection. An antibody to ICAM-1 inhibited HRV-14 infection of epithelial cells and abolished H2O2-induced increases in G and [3H]mannitol flux in IL-1beta -pretreated epithelial cells with HRV-14 infection. These results suggest that rhinovirus infection may reduce barrier functions in the airway epithelium in association with upregulation of ICAM-1 expression.




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