help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by BASKURT, O. K.
Right arrow Articles by MEISELMAN, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by BASKURT, O. K.
Right arrow Articles by MEISELMAN, H. J.

Am. J. Respir. Crit. Care Med., Volume 157, Number 2, February 1998, 421-427

Red Blood Cell Deformability in Sepsis

OGUZ K. BASKURT, DAVID GELMONT, and HERBERT J. MEISELMAN

Department of Physiology and Biophysics and Division of Pulmonary and Critical Care Medicine, University of Southern California School of Medicine, Los Angeles, California

The microcirculatory disturbances in sepsis have prompted micropore bulk-filtration studies of red blood cell (RBC) mechanical behavior (i.e., deformability). However, these prior reports may not solely reflect RBC behavior because of possible white blood cell (WBC) occlusion of the filter pores. The present study was designed to examine RBC mechanical alterations in human and experimental sepsis using techniques that are not affected by WBC artifacts. RBC were obtained from adult patients with sepsis and from healthy control donors. RBC were also obtained from Swiss-albino rats in which experimental sepsis was induced via cecal ligation-puncture. Red cell mechanical behavior was tested using a computerized micropore filtration system (CTA) and a laser-diffraction shearing device (LORCA); the latter provides the extent of RBC deformation at various stresses and the time constant for RBC shape recovery. Salient findings include: (1) for human RBC, significantly decreased deformability at fluid shear stresses < 5 Pa (LORCA) yet no differences from control with the CTA; (2) for rat RBC in experimental sepsis, significant decreases of deformability and shape-recovery time constant (LORCA) but no differences with the CTA. We conclude that RBC deformability is reduced in sepsis but that micropore bulk-filtration methods may not be appropriate for detecting these changes.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. R. Condon, J. E. Kim, E. A. Deitch, G. W. Machiedo, and Z. Spolarics
Appearance of an erythrocyte population with decreased deformability and hemoglobin content following sepsis
Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2177 - H2184.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. M. Bateman, J. E. Jagger, M. D. Sharpe, M. L. Ellsworth, S. Mehta, and C. G. Ellis
Erythrocyte deformability is a nitric oxide-mediated factor in decreased capillary density during sepsis
Am J Physiol Heart Circ Physiol, June 1, 2001; 280(6): H2848 - H2856.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. Armour, K. Tyml, D. Lidington, and J. X. Wilson
Ascorbate prevents microvascular dysfunction in the skeletal muscle of the septic rat
J Appl Physiol, March 1, 2001; 90(3): 795 - 803.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1998 American Thoracic Society 		  CCM abstracts