Am. J. Respir. Crit. Care Med., Volume 156, Number 4, October 1997, 1073-1081

Reduced Lipid Peroxidation and Ischemia-Reperfusion Injury after Lung Transplantation Using Low-Potassium Dextran Solution for Lung Preservation

FUMIO SAKAMAKI, HANS HOFFMANN, CHRISTIAN MÜLLER, HENDRIK DIENEMANN, KONRAD MESSMER, and FRIEDRICH W. SCHILDBERG

Department of Surgery and Institute for Surgical Research, Klinikum Grosshadern, Ludwig-Maximilians-Universität München, Munich, Germany

Ischemia-reperfusion injury is one of the significant problems in clinical lung transplantation. We investigated the effect of lung preservation with Euro-Collins solution (EC group) or low-potassium dextran solution (LPD group) on lipid peroxidation and ischemia-reperfusion injury in a pig model of lung allotransplantation. The donor lungs were preserved at 4° C for 18 h. Left-sided single lung transplantation was performed, followed by 6 h of reperfusion. Lipid peroxidation was measured as thiobarbituric acid-reactive materials (TBARM) in bronchoalveolar lavage (BAL) fluid and effluent solutions from pulmonary artery (Effluent). After 18 h of ischemia, the LPD group showed lower TBARM in BAL and Effluent than the EC group (p < 0.05). After ischemia plus reperfusion, lung wet-to-dry weight ratios and TBARM levels in BAL in the LPD group were lower than those of the EC group (p < 0.05). Lung wet-to-dry weight ratios correlated with TBARM levels in BAL (p < 0.05, r = 0.50). We conclude lipid peroxidation in BAL and Effluent may reflect the degree of ischemia-reperfusion injury, and lung preservation with LPD can reduce lipid peroxidation and lung injury as compared with EC.

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