help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by SIELENKÄMPER, A. W.
Right arrow Articles by SIBBALD, W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SIELENKÄMPER, A. W.
Right arrow Articles by SIBBALD, W. J.

Am. J. Respir. Crit. Care Med., Volume 156, Number 4, October 1997, 1066-1072

Diaspirin Crosslinked Hemoglobin Improves Systemic Oxygen Uptake in Oxygen Supply-dependent Septic Rats

ANDREAS W. SIELENKÄMPER, IAN H. CHIN-YEE, CLAUDIO M. MARTIN, and WILLIAM J. SIBBALD

A. C. Burton Vascular Biology Laboratory, Victoria Hospital Research Institute and University of Western Ontario, London, Ontario

Diaspirin crosslinked hemoglobin (DCLHb) is a cell-free hemoglobin derived from human erythrocytes. DCLHb has been shown to improve blood flow to vital organs in healthy and septic animals. In this study, we determined the efficacy of DCLHb by comparing its effect on systemic O2 uptake to freshly stored and aged red blood cells (RBCs) in septic rats. Twenty-four hours after induction of sepsis by cecal ligation and perforation, O2 supply dependency was created by isovolemic hemodilution with rat plasma. In O2 supply dependency, rats were randomized to receive an exchange transfusion of 7.5 ml "fresh" RBCs (stored < 6 d; Hct: 70%), "fresh" diluted RBCs (stored < 6 d; Hct: 30%), "old" RBCs (stored 28 to 35 d; Hct: 70%), or DCLHb (Hb: 100 g/L). We found, that survival following O2 supply dependency and transfusion with old RBCs was poor (33% versus 91.7% in the other groups; p < 0.01), precluding further analysis of post-transfusion data from this group. Systemic O2 uptake increased in all remaining groups (p < 0.001), while systemic O2 delivery increased with "fresh" RBCs (p < 0.0001) and "fresh" diluted RBCs (p < 0.05) but not with DCLHb. Systemic O2 extraction increased with DCLHb as compared to baseline (p < 0.05) and to the other groups (p < 0.0001). Improved tissue oxygenation was associated with an increase in blood pressure and a fall in arterial lactate in all groups. We conclude that transfusion of DCLHb or "fresh" RBCs was efficacious at increasing systemic O2 uptake in O2 supply-dependent, septic rats.




This article has been cited by other articles:


Home page
 Anesth. Analg.Home page
L. G. Fischer, J. H. Hilpert, H. Freise, D. Wendholt, H. Van Aken, and A. W. Sielenkamper
Bradykinin-Induced Pulmonary Vasoconstriction Is Time and Inducible Nitric Oxide Synthase Dependent in a Peritonitis Sepsis Model
Anesth. Analg., September 1, 2004; 99(3): 864 - 871.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
S. Suttner, S. N. Piper, B. Kumle, K. Lang, K. D. Rohm, F. Isgro, and J. Boldt
The Influence of Allogeneic Red Blood Cell Transfusion Compared with 100% Oxygen Ventilation on Systemic Oxygen Transport and Skeletal Muscle Oxygen Tension After Cardiac Surgery
Anesth. Analg., July 1, 2004; 99(1): 2 - 11.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
J.-F. Hardy, S. Belisle, P. Van der Linden, C. Cothren, E. E. Moore, and J. L. Johnson
Combined Blood Substitute and Erythropoietin Therapy in a Severely Injured Jehovah's Witness
N. Engl. J. Med., August 29, 2002; 347(9): 696 - 697.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. V. d. LINDEN, S. DE HERT, S. BELISLE, F. DE GROOTE, N. MATHIEU, S. D'EUGENIO, V. JULIEN, C. HUYNH, and C. MELOT
Comparative Effects of Red Blood Cell Transfusion and Increasing Blood Flow on Tissue Oxygenation in Oxygen Supply-dependent Conditions
Am. J. Respir. Crit. Care Med., June 1, 2001; 163(7): 1605 - 1608.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. Creteur, H. Zhang, D. De Backer, Q. Sun, and J.-L. Vincent
Diaspirin cross-linked hemoglobin improves oxygen extraction capabilities in endotoxic shock
J Appl Physiol, October 1, 2000; 89(4): 1437 - 1444.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. W. Sielenkamper, P. Yu, O. Eichelbronner, T. MacDonald, C. M. Martin, I. H. Chin-Yee, and W. J. Sibbald
Diaspirin cross-linked Hb and norepinephrine prevent the sepsis-induced increase in critical O2 delivery
Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H1922 - H1930.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1997 American Thoracic Society