Am. J. Respir. Crit. Care Med., Volume 156, Number 3, September 1997, 855-861

Spiking Survanta with Synthetic Surfactant Peptides Improves Oxygenation in Surfactant-deficient Rats

FRANS J. WALTHER, JOSÉ HERNÁNDEZ-JUVIEL, ROBERTA BRUNI, and ALAN J. WARING

Department of Pediatrics, Charles R. Drew University of Medicine and Science; and Perinatal Laboratories, Harbor-UCLA Research and Education Institute, University of California Los Angeles School of Medicine, Los Angeles, California

The hypothesis that the in vivo function of Survanta (Beractant) can be improved by supplementation with synthetic surfactant peptides B and C was tested in a surfactant-deficient rat model. Full length surfactant protein-B (SP-B_1-78) (B) and palmitoylated surfactant protein-C (SP-C_1-35) (C), and synthetic KL₄ peptide were added to Survanta after extraction, creating extracted Survanta (ES) with 1% B, 2% B, and 2% B plus 1% C, or mixed with Survanta without extraction, creating modified Survanta (S) with 2% B, 2% B plus 1% C, and 2% KL₄. Adult rats were ventilated with 100% oxygen, tidal volumes (VT) of 7.5 ml/kg and a rate of 60/min, and were lavaged until the Pa_O2 dropped below 80 mm Hg, when 100 mg/kg of surfactant was instilled. After 15 to 60 min of ventilation, pressure-volume (P-V) curves were generated in situ. Instillation of ES or S with 2% B plus 1% C led to the greatest increase in oxygenation, closely followed by ES and S with 2% B, and more distantly by S plus 2% KL₄. TLC was comparable among the ES and S groups, but greater than that of air-placebo controls. These data suggest that spiking of Survanta with synthetic SP-B and SP-C increased oxygenation more effectively than B or KL₄ alone in this surfactant-deficient rat model.

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