help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by HUMBERT, M.
Right arrow Articles by DURHAM, S. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by HUMBERT, M.
Right arrow Articles by DURHAM, S. R.

Am. J. Respir. Crit. Care Med., Volume 156, Number 3, September 1997, 704-708

Relationship between IL-4 and IL-5 mRNA Expression and Disease Severity in Atopic Asthma

MARC HUMBERT, CHRISTOPHER J. CORRIGAN, PATRICK KIMMITT, STEPHEN J. TILL, A. BARRY KAY, and STEPHEN R. DURHAM

Allergy and Clinical Immunology, Imperial College School of Medicine at the National Heart and Lung Institute, London, United Kingdom

Atopic asthma is characterized by chronic inflammation of the bronchial mucosa in which eosinophil- and immunoglobulin E (IgE)-dependent mechanisms are believed to be prominent. Therefore, specific proeosinophilic mediators such as interleukin (IL)-5 and essential cofactors for IgE switching in B-lymphocytes such as IL-4 could play a pivotal role in asthma. However, the exact role that individual inflammatory mediators play in the development of the disease in humans is still unknown. Using semiquantitative reverse transcriptase-polymerase chain reaction amplification in bronchial biopsies from 10 atopic asthmatics, we have tested the hypothesis that IL-4 and IL-5 mRNA expression relative to beta -actin mRNA correlates with validated indicators of disease severity. IL-4 and IL-5 mRNA copies relative to beta -actin mRNA were detected in bronchial biopsies from atopic asthmatics. The numbers of IL-5 mRNA copies relative to beta -actin mRNA correlated with disease severity assessed by the Aas asthma score (r = 0.70, p = 0.01), baseline FEV1 (r = -0.94, p = 0.001), baseline peak expiratory flow rate (r = -0.77, p = 0.01), peak expiratory flow rate variability over 2 wk (r = 0.69, p = 0.028), and the histamine PC20 (r = -0.72, p = 0.018). Conversely, the numbers of IL-4 mRNA copies relative to beta -actin mRNA did not correlate with asthma severity, but they positively correlated with total serum IgE concentrations (r = -0.90, p = 0.001). Our present results support the concept that IL-5 may determine asthma clinical expression and severity, and by inference they support the development of IL-5 targeted therapies.




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
P. Flood-Page, C. Swenson, I. Faiferman, J. Matthews, M. Williams, L. Brannick, D. Robinson, S. Wenzel, W. Busse, T. T. Hansel, et al.
A Study to Evaluate Safety and Efficacy of Mepolizumab in Patients with Moderate Persistent Asthma
Am. J. Respir. Crit. Care Med., December 1, 2007; 176(11): 1062 - 1071.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
C. Bergeron and L.-P. Boulet
Structural changes in airway diseases: characteristics, mechanisms, consequences, and pharmacologic modulation.
Chest, April 1, 2006; 129(4): 1068 - 1087.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
E Truyen, L Coteur, E Dilissen, L Overbergh, L J Dupont, J L Ceuppens, and D M A Bullens
Evaluation of airway inflammation by quantitative Th1/Th2 cytokine mRNA measurement in sputum of asthma patients
Thorax, March 1, 2006; 61(3): 202 - 208.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
C. Buttner, A. Lun, T. Splettstoesser, G. Kunkel, and H. Renz
Monoclonal anti-interleukin-5 treatment suppresses eosinophil but not T-cell functions
Eur. Respir. J., May 1, 2003; 21(5): 799 - 803.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
V Brown, T J Warke, M D Shields, and M Ennis
T cell cytokine profiles in childhood asthma
Thorax, April 1, 2003; 58(4): 311 - 316.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. T. Flood-Page, A. N. Menzies-Gow, A. B. Kay, and D. S. Robinson
Eosinophil's Role Remains Uncertain as Anti-Interleukin-5 only Partially Depletes Numbers in Asthmatic Airway
Am. J. Respir. Crit. Care Med., January 15, 2003; 167(2): 199 - 204.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
W. M. Abraham
Of Mice and Men
Am. J. Respir. Cell Mol. Biol., January 1, 2003; 28(1): 1 - 4.
[Full Text] [PDF]

Home page
 ThoraxHome page
E L J van Rensen, R G Stirling, J Scheerens, K Staples, P J Sterk, P J Barnes, and K F Chung
Evidence for systemic rather than pulmonary effects of interleukin-5 administration in asthma
Thorax, December 1, 2001; 56(12): 935 - 940.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. G. STIRLING, E. L. J. VAN RENSEN, P. J. BARNES, and K. FAN CHUNG
Interleukin-5 Induces CD34+ Eosinophil Progenitor Mobilization and Eosinophil CCR3 Expression in Asthma
Am. J. Respir. Crit. Care Med., October 15, 2001; 164(8): 1403 - 1409.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. J. Staples, M. Bergmann, K. Tomita, M. D. Houslay, I. McPhee, P. J. Barnes, M. A. Giembycz, and R. Newton
Adenosine 3',5'-Cyclic Monophosphate (cAMP)-Dependent Inhibition of IL-5 from Human T Lymphocytes Is Not Mediated by the cAMP-Dependent Protein Kinase A
J. Immunol., August 15, 2001; 167(4): 2074 - 2080.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
K. OSHIKAWA, K. KUROIWA, K. TAGO, H. IWAHANA, K. YANAGISAWA, S. OHNO, S.-I. TOMINAGA, and Y. SUGIYAMA
Elevated Soluble ST2 Protein Levels in Sera of Patients with Asthma with an Acute Exacerbation
Am. J. Respir. Crit. Care Med., July 15, 2001; 164(2): 277 - 281.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. C. LAPORTE, P. E. MOORE, S. BARALDO, M.-H. JOUVIN, T. L. CHURCH, I. N. SCHWARTZMAN, R. A. PANETTIERI Jr, J.-P. KINET, S. A. SHORE, and S. A. SHORE
Direct Effects of Interleukin-13 on Signaling Pathways for Physiological Responses in Cultured Human Airway Smooth Muscle Cells
Am. J. Respir. Crit. Care Med., July 1, 2001; 164(1): 141 - 148.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
E M Glare, M Divjak, M J Bailey, and E H Walters
The usefulness of competitive PCR: airway gene expression of IL-5, IL-4, IL-4{delta}2, IL-2, and IFN{gamma} in asthma
Thorax, July 1, 2001; 56(7): 541 - 548.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. Van Wauwe, F. Aerts, M. Cools, F. Deroose, E. Freyne, J. Goossens, B. Hermans, J. Lacrampe, H. Van Genechten, F. Van Gerven, et al.
Identification of R146225 as a Novel, Orally Active Inhibitor of Interleukin-5 Biosynthesis
J. Pharmacol. Exp. Ther., November 1, 2000; 295(2): 655 - 661.
[Abstract] [Full Text]

Home page
 BloodHome page
J. Tavernier, J. Van der Heyden, A. Verhee, G. Brusselle, X. Van Ostade, J. Vandekerckhove, J. North, S. M. Rankin, A. B. Kay, and D. S. Robinson
Interleukin 5 regulates the isoform expression of its own receptor alpha -subunit
Blood, March 1, 2000; 95(5): 1600 - 1607.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Mori, O. Kaminuma, K. Miyazawa, K. Ogawa, H. Okudaira, and K. Akiyama
p38 Mitogen-Activated Protein Kinase Regulates Human T Cell IL-5 Synthesis
J. Immunol., November 1, 1999; 163(9): 4763 - 4771.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. P. McLane, A. Haczku, M. van de Rijn, C. Weiss, V. Ferrante, D. MacDonald, J.-C. Renauld, N. C. Nicolaides, K. J. Holroyd, and R. C. Levitt
Interleukin-9 Promotes Allergen-Induced Eosinophilic Inflammation and Airway Hyperresponsiveness in Transgenic Mice
Am. J. Respir. Cell Mol. Biol., November 1, 1998; 19(5): 713 - 720.
[Abstract] [Full Text]

Home page
 ThoraxHome page
J. Temelkovski, S. P Hogan, D. P Shepherd, P. S Foster, and R. K Kumar
An improved murine model of asthma: selective airway inflammation, epithelial lesions and increased methacholine responsiveness following chronic exposure to aerosolised allergen
Thorax, October 1, 1998; 53(10): 849 - 856.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1997 American Thoracic Society