help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by TAKATA, M.
Right arrow Articles by MIYASAKA, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by TAKATA, M.
Right arrow Articles by MIYASAKA, K.

Am. J. Respir. Crit. Care Med., Volume 156, Number 1, July 1997, 272-279

Intraalveolar Expression of Tumor Necrosis Factor-alpha Gene during Conventional and High-Frequency Ventilation

MASAO TAKATA, JUN ABE, HIROYUKI TANAKA, YOSHIHIRO KITANO, SHOUZABUROH DOI, TAKAO KOHSAKA, and KATSUYUKI MIYASAKA

Divisions of Pathophysiology and Immunology, National Children's Medical Research Center, Tokyo, Japan

The effects of conventional mechanical ventilation (CMV) and high-frequency oscillatory ventilation (HFO) on intraalveolar expression of the tumor necrosis factor-alpha (TNF-alpha ) gene were studied in surfactant-depleted rabbits. After lung lavage with saline, 13 rabbits were administered either CMV (n = 6) or HFO (n = 7) for 1 h at an FIO2 of 1.0 and a mean airway pressure of 13 cm H2O. Lung lavage was then repeated. The rabbits' RNA was extracted from the lavage cells, and mRNA for TNF-alpha was quantitated by reverse-transcription polymerase chain reaction using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as an internal standard. At 1 h of ventilation, PaO2 was slightly lower with CMV than HFO, while lavage cell counts and cytology were similar between the two groups. The ratio of TNF-alpha mRNA to GAPDH mRNA increased with CMV (control, 0.48 ± 0.04 [SE] versus 1 h, 1.02 ± 0.14, p < 0.01) but did not change with HFO (0.55 ± 0.07 versus 0.73 ± 0.09). In a separate series of experiments, ten surfactant-depleted rabbits continued to be ventilated for 4 h either by CMV (n = 5) or HFO (n = 5). Conventional mechanical ventilation resulted in a progressive hypoxemia, decreased lung compliance, increased number of neutrophils in lung lavage fluid, and substantial morphological changes including hyaline membrane formation and neutrophil accumulation, whereas HFO was associated with minimal changes in such physiological and pathological abnormalities. These results suggest that activation of alveolar macrophages and production of proinflammatory cytokines may play a pivotal role in the early stage of ventilator-induced lung injury, and that ventilator mode (CMV or HFO) substantially modulates macrophage activation and hence the degree of lung injury.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. A. Nonas, L. M. Vinasco, S. F. Ma, J. R. Jacobson, A. A. Desai, S. M. Dudek, C. Flores, P. M. Hassoun, L. Sam, S. Q. Ye, et al.
Use of consomic rats for genomic insights into ventilator-associated lung injury
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L292 - L302.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. R. Wilson, M. E. Goddard, K. P. O'Dea, S. Choudhury, and M. Takata
Differential roles of p55 and p75 tumor necrosis factor receptors on stretch-induced pulmonary edema in mice
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L60 - L68.
[Abstract] [Full Text] [PDF]

Home page
 PediatricsHome page
P. Truffert, J. Paris-Llado, B. Escande, J.-F. Magny, G. Cambonie, E. Saliba, G. Thiriez, V. Zupan-Simunek, T. Blanc, J.-C. Roze, et al.
Neuromotor Outcome at 2 Years of Very Preterm Infants Who Were Treated With High-Frequency Oscillatory Ventilation or Conventional Ventilation for Neonatal Respiratory Distress Syndrome
Pediatrics, April 1, 2007; 119(4): e860 - e865.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
J. A. Frank, P. E. Parsons, and M. A. Matthay
Pathogenetic Significance of Biological Markers of Ventilator-Associated Lung Injury in Experimental and Clinical Studies
Chest, December 1, 2006; 130(6): 1906 - 1914.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. H. Kim, M. H. Suk, D. W. Yoon, S. H. Lee, G. Y. Hur, K. H. Jung, H. C. Jeong, S. Y. Lee, S. Y. Lee, I. B. Suh, et al.
Inhibition of matrix metalloproteinase-9 prevents neutrophilic inflammation in ventilator-induced lung injury
Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L580 - L587.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
N. G. Hall, Y. Liu, J. M. Hickman-Davis, G. C. Davis, C. Myles, E. J. Andrews, S. Matalon, and J. D. Lang Jr.
Bactericidal Function of Alveolar Macrophages in Mechanically Ventilated Rabbits
Am. J. Respir. Cell Mol. Biol., June 1, 2006; 34(6): 719 - 726.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Miyao, Y. Suzuki, K. Takeshita, H. Kudo, M. Ishii, R. Hiraoka, K. Nishio, T. Tamatani, S. Sakamoto, M. Suematsu, et al.
Various adhesion molecules impair microvascular leukocyte kinetics in ventilator-induced lung injury
Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1059 - L1068.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Tsuchida, D. Engelberts, M. Roth, C. McKerlie, M. Post, and B. P. Kavanagh
Continuous positive airway pressure causes lung injury in a model of sepsis
Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L554 - L564.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. N. Tremblay and A. S. Slutsky
Pathogenesis of ventilator-induced lung injury: trials and tribulations
Am J Physiol Lung Cell Mol Physiol, April 1, 2005; 288(4): L596 - L598.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. R. Wilson, S. Choudhury, and M. Takata
Pulmonary inflammation induced by high-stretch ventilation is mediated by tumor necrosis factor signaling in mice
Am J Physiol Lung Cell Mol Physiol, April 1, 2005; 288(4): L599 - L607.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. L. Farias, D. S. Faffe, D. G. Xisto, M. C. E. Santana, R. Lassance, L. F. M. Prota, M. B. Amato, M. M. Morales, W. A. Zin, and P. R. M. Rocco
Positive end-expiratory pressure prevents lung mechanical stress caused by recruitment/derecruitment
J Appl Physiol, January 1, 2005; 98(1): 53 - 61.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Choudhury, M. R. Wilson, M. E. Goddard, K. P. O'Dea, and M. Takata
Mechanisms of early pulmonary neutrophil sequestration in ventilator-induced lung injury in mice
Am J Physiol Lung Cell Mol Physiol, November 1, 2004; 287(5): L902 - L910.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
H. Wrigge, U. Uhlig, J. Zinserling, E. Behrends-Callsen, G. Ottersbach, M. Fischer, S. Uhlig, and C. Putensen
The Effects of Different Ventilatory Settings on Pulmonary and Systemic Inflammatory Responses During Major Surgery
Anesth. Analg., March 1, 2004; 98(3): 775 - 781.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
U. Uhlig, H. Fehrenbach, R. A. Lachmann, T. Goldmann, B. Lachmann, E. Vollmer, and S. Uhlig
Phosphoinositide 3-OH Kinase Inhibition Prevents Ventilation-induced Lung Cell Activation
Am. J. Respir. Crit. Care Med., January 15, 2004; 169(2): 201 - 208.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. M. Steinberg, H. J. Schiller, J. M. Halter, L. A. Gatto, H.-M. Lee, L. A. Pavone, and G. F. Nieman
Alveolar Instability Causes Early Ventilator-induced Lung Injury Independent of Neutrophils
Am. J. Respir. Crit. Care Med., January 1, 2004; 169(1): 57 - 63.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. G. Laffey, R. P. Jankov, D. Engelberts, A. K. Tanswell, M. Post, T. Lindsay, J. B. Mullen, A. Romaschin, D. Stephens, C. McKerlie, et al.
Effects of Therapeutic Hypercapnia on Mesenteric Ischemia-Reperfusion Injury
Am. J. Respir. Crit. Care Med., December 1, 2003; 168(11): 1383 - 1390.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. R. Wilson, S. Choudhury, M. E. Goddard, K. P. O'Dea, A. G. Nicholson, and M. Takata
High tidal volume upregulates intrapulmonary cytokines in an in vivo mouse model of ventilator-induced lung injury
J Appl Physiol, October 1, 2003; 95(4): 1385 - 1393.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
S. Fernandez-Serrano, J. Dorca, M. Coromines, J. Carratala, F. Gudiol, and F. Manresa
Molecular Inflammatory Responses Measured in Blood of Patients with Severe Community-Acquired Pneumonia
Clin. Vaccine Immunol., September 1, 2003; 10(5): 813 - 820.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
J-D. Ricard, D. Dreyfuss, and G. Saumon
Ventilator-induced lung injury
Eur. Respir. J., August 1, 2003; 22(42_suppl): 2s - 9s.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. Dreyfuss, J.-D. Ricard, and G. Saumon
On the Physiologic and Clinical Relevance of Lung-borne Cytokines during Ventilator-induced Lung Injury
Am. J. Respir. Crit. Care Med., June 1, 2003; 167(11): 1467 - 1471.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. M. Waters, P. H. S. Sporn, M. Liu, and J. J. Fredberg
Cellular biomechanics in the lung
Am J Physiol Lung Cell Mol Physiol, September 1, 2002; 283(3): L503 - L509.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. E. Sinclair, D. A. Kregenow, W. J. E. Lamm, I. R. Starr, E. Y. Chi, and M. P. Hlastala
Hypercapnic Acidosis Is Protective in an In Vivo Model of Ventilator-induced Lung Injury
Am. J. Respir. Crit. Care Med., August 1, 2002; 166(3): 403 - 408.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Tasiemski, H. Hammad, F. Vandenbulcke, C. Breton, T. J. Bilfinger, J. Pestel, and M. Salzet
Presence of chromogranin-derived antimicrobial peptides in plasma during coronary artery bypass surgery and evidence of an immune origin of these peptides
Blood, June 28, 2002; 100(2): 553 - 559.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Y. Imai, S. Nakagawa, Y. Ito, T. Kawano, A. S. Slutsky, and K. Miyasaka
Comparison of lung protection strategies using conventional and high-frequency oscillatory ventilation
J Appl Physiol, October 1, 2001; 91(4): 1836 - 1844.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
J.-D. Ricard and D. Dreyfuss
Cytokines During Ventilator-Induced Lung Injury: A Word of Caution
Anesth. Analg., August 1, 2001; 93(2): 251 - 252.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J.-D. RICARD, D. DREYFUSS, and G. SAUMON
Production of Inflammatory Cytokines in Ventilator-Induced Lung Injury: A Reappraisal
Am. J. Respir. Crit. Care Med., April 1, 2001; 163(5): 1176 - 1180.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
H.-D. HELD, S. BOETTCHER, L. HAMANN, and S. UHLIG
Ventilation-Induced Chemokine and Cytokine Release Is Associated with Activation of Nuclear Factor-{kappa}B and Is Blocked by Steroids
Am. J. Respir. Crit. Care Med., March 1, 2001; 163(3): 711 - 716.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
H. Imanaka, M. Shimaoka, N. Matsuura, M. Nishimura, N. Ohta, and H. Kiyono
Ventilator-Induced Lung Injury Is Associated with Neutrophil Infiltration, Macrophage Activation, and TGF-{beta}1 mRNA Upregulation in Rat Lungs
Anesth. Analg., February 1, 2001; 92(2): 428 - 436.
[Abstract] [Full Text] [PDF]

Home page
 PediatricsHome page
G. Moriette, J. Paris-Llado, H. Walti, B. Escande, J.-F. Magny, G. Cambonie, G. Thiriez, S. Cantagrel, T. Lacaze-Masmonteil, L. Storme, et al.
Prospective Randomized Multicenter Comparison of High-Frequency Oscillatory Ventilation and Conventional Ventilation in Preterm Infants of Less Than 30 Weeks With Respiratory Distress Syndrome
Pediatrics, February 1, 2001; 107(2): 363 - 372.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. G. LAFFEY, M. TANAKA, D. ENGELBERTS, X. LUO, S. YUAN, A. KEITH TANSWELL, M. POST, T. LINDSAY, and B. P. KAVANAGH
Therapeutic Hypercapnia Reduces Pulmonary and Systemic Injury following In Vivo Lung Reperfusion
Am. J. Respir. Crit. Care Med., December 1, 2000; 162(6): 2287 - 2294.
[Abstract] [Full Text]

Home page
 PediatricsHome page
D. W. Thibeault, S. M. Mabry, I. I. Ekekezie, and W. E. Truog
Lung Elastic Tissue Maturation and Perturbations During the Evolution of Chronic Lung Disease
Pediatrics, December 1, 2000; 106(6): 1452 - 1459.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
B. A. YODER, T. SILER-KHODR, V. T. WINTER, and J. J. COALSON
High-frequency Oscillatory Ventilation . Effects on Lung Function, Mechanics, and Airway Cytokines in the Immature Baboon Model for Neonatal Chronic Lung Disease
Am. J. Respir. Crit. Care Med., November 1, 2000; 162(5): 1867 - 1876.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. Mourgeon, N. Isowa, S. Keshavjee, X. Zhang, A. S. Slutsky, and M. Liu
Mechanical stretch stimulates macrophage inflammatory protein-2 secretion from fetal rat lung cells
Am J Physiol Lung Cell Mol Physiol, October 1, 2000; 279(4): L699 - L706.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
J. A. Krishnan and R. G. Brower
High-Frequency Ventilation for Acute Lung Injury and ARDS
Chest, September 1, 2000; 118(3): 795 - 807.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. DURAND, M. BACHELET, F. BRUNET, M. J. RICHARD, J.-F. DHAINAUT, J. DALL'AVA, and B. S. POLLA
Inducibility of the 70 kD Heat Shock Protein in Peripheral Blood Monocytes Is Decreased in Human Acute Respiratory Distress Syndrome and Recovers Over Time
Am. J. Respir. Crit. Care Med., January 1, 2000; 161(1): 286 - 292.
[Abstract] [Full Text]

Home page
 Anesth. Analg.Home page
N. Kotani, H. Hashimoto, D. I. Sessler, T. Yasuda, T. Ebina, M. Muraoka, and A. Matsuki
Expression of Genes for Proinflammatory Cytokines in Alveolar Macrophages During Propofol and Isoflurane Anesthesia
Anesth. Analg., November 1, 1999; 89(5): 1250 - 1250.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Y. Imai, T. Kawano, S. Iwamoto, S. Nakagawa, M. Takata, and K. Miyasaka
Intratracheal anti-tumor necrosis factor-alpha antibody attenuates ventilator-induced lung injury in rabbits
J Appl Physiol, August 1, 1999; 87(2): 510 - 515.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. CHIUMELLO, G. PRISTINE, and A. S. SLUTSKY
Mechanical Ventilation Affects Local and Systemic Cytokines in an Animal Model of Acute Respiratory Distress Syndrome
Am. J. Respir. Crit. Care Med., July 1, 1999; 160(1): 109 - 116.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Pugin, I. Dunn, P. Jolliet, D. Tassaux, J.-L. Magnenat, L. P. Nicod, and J.-C. Chevrolet
Activation of human macrophages by mechanical ventilation in vitro
Am J Physiol Lung Cell Mol Physiol, December 1, 1998; 275(6): L1040 - L1050.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. S. SLUTSKY and L. N. TREMBLAY
Multiple System Organ Failure . Is Mechanical Ventilation a Contributing Factor?
Am. J. Respir. Crit. Care Med., June 1, 1998; 157(6): 1721 - 1725.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. DREYFUSS and G. SAUMON
Ventilator-induced Lung Injury . Lessons from Experimental Studies
Am. J. Respir. Crit. Care Med., January 1, 1997; 157(1): 294 - 323.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1997 American Thoracic Society