Am. J. Respir. Crit. Care Med., Volume 156, Number 1, July 1997, 140-145

Increased Superoxide Production during Fatigue in the Perfused Rat Diaphragm

RALPH C. KOLBECK, ZHI-WU SHE, LEIGH A. CALLAHAN, and AND THOMAS M. NOSEK

Muscle Cell Biology Research Group, Department of Medicine, Department of Physiology/Endocrinology, Medical College of Georgia, Augusta, Georgia

This study test the hypothesis that a temporal relationship exists between the production of superoxide anion (O₂) and the contractile activity of perfused rat diaphragm. O₂ levels were determined minute to minute by measuring the reduction of cytochrome c in the perfusate as the diaphragms were subjected to various levels of contractile activity. After equilibrating at low contractile rates (one 500 ms 80 Hz train/min), diaphragms were fatigued by increasing their contractile activity for 5 min (one 500 ms 80 Hz train/s) and then allowed to recover for 30 min (one 500 ms 80 Hz train/min). During equilibration, diaphragms did not produce O₂ above the background level measured in the presence of superoxide dismutase (SOD). Within the first minute of fatigue-inducing stimulation, however, the rate of O₂ production increased to 0.70 ± 0.17 nmol/min and remained elevated until the recovery period when production returned towards baseline. SOD blocked this stimulation-related increase of O₂. Tension (± SOD) fell to 12% of the control value during the fatigue-inducing stimulation. During recovery the contractile response returned to 51% of control, indicating long-lasting effects on the contractile machinery. SOD did not limit fatigue or improve recovery, probably because it is a large protein that cannot cross cell membranes and protect the cells by scavenging O₂ at its site of production.

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