help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Song, P.
Right arrow Articles by Brusasco, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Song, P.
Right arrow Articles by Brusasco, V.

Am. J. Respir. Crit. Care Med., Vol 155, No. 4, 04 1997, 1230-1234.

Allergen challenge of passively sensitized human bronchi alters M2 and beta2 receptor function

P Song, M Milanese, E Crimi, K Rehder and V Brusasco
Cattedra di Fisiopatologia Respiratoria, Dipartimento di Scienze Motorie e Riabilitative, Facolta di Medicina e Chirurgia, Universita di Genova, Italy.

We investigated whether antigen challenge may alter M2 and beta2 receptor function in isolated passively sensitized human bronchi. Bronchial rings (2-4 mm internal diameter) were obtained from 12 patients. Passive sensitization was induced by serum containing high IgE levels to Dermatophagoides pteronyssinus and Dermatophagoides farinae. Rings from six patients were used to study M2 receptor function by incubating with cumulatively increasing concentrations (10(- 8) M to 10(-4) M) of pilocarpine and applying electric field stimulation (EFS) at 24 Hz. The rings from the other six patients were used to study beta2 receptor function by precontracting with carbachol 10(-6) M and adding cumulatively salbutamol (10(-9) M to 10(-4) M). Additional rings from these patients were used to determine whether dysfunction occurred distal to cAMP production by precontracting with carbachol 10(-6) M and adding cumulatively theophylline (10(-9) M to 10(-4) M). The attenuation of EFS-induced force by pilocarpine and the relaxation of carbachol-precontracted rings by salbutamol were less in challenged than in control and sensitized rings. No difference between challenged, sensitized, and control rings was observed with theophylline. We conclude that allergen challenge in passively sensitized isolated human bronchial rings may result in M2 and beta2 receptor dysfunction without involving mechanisms distal to cAMP formation. It appears that products from inflammatory cells recruited from blood are not necessary for this receptor dysfunction.


This article has been cited by other articles:


Home page
 Therapeutic Advances in Respiratory DiseaseHome page
M. Baroffio, E. Crimi, and V. Brusasco
Review: Airway smooth muscle as a model for new investigative drugs in asthma
Therapeutic Advances in Respiratory Disease, June 1, 2008; 2(3): 129 - 139.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Wang, V. Pozzato, G. Turato, A. Madamanchi, T. M. Murphy, and P. Chitano
Reduced spontaneous relaxation in immature guinea pig airway smooth muscle is associated with increased prostanoid release
Am J Physiol Lung Cell Mol Physiol, May 1, 2008; 294(5): L964 - L973.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. Chitano, L. Wang, S. N. Mason, R. L. Auten, E. N. Potts, W. M. Foster, A. Sturrock, T. P. Kennedy, J. R. Hoidal, and T. M. Murphy
Airway smooth muscle relaxation is impaired in mice lacking the p47phox subunit of NAD(P)H oxidase
Am J Physiol Lung Cell Mol Physiol, January 1, 2008; 294(1): L139 - L148.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
V. Brusasco and R. Pellegrino
Invited Review: Complexity of factors modulating airway narrowing in vivo: relevance to assessment of airway hyperresponsiveness
J Appl Physiol, September 1, 2003; 95(3): 1305 - 1313.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Brichetto, M. Milanese, P. Song, M. Patrone, E. Crimi, K. Rehder, and V. Brusasco
Beclomethasone rapidly ablates allergen-induced beta 2- adrenoceptor pathway dysfunction in human isolated bronchi
Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L133 - L139.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. L. BLACK, M. ROTH, J. LEE, S. CARLIN, and P. R. A. JOHNSON
Mechanisms of Airway Remodeling . Airway Smooth Muscle
Am. J. Respir. Crit. Care Med., November 15, 2001; 164(10): S63 - 66.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
D.C. Grootendorst, P.J. Sterk, and H.G.M. Heijerman
Effect of oral prednisolone on the bronchoprotective effect of formoterol in patients with persistent asthma
Eur. Respir. J., March 1, 2001; 17(3): 374 - 379.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. Song, M. Milanese, E. Crimi, S. Bruzzone, E. Zocchi, K. Rehder, and V. Brusasco
Gs protein dysfunction in allergen-challenged human isolated passively sensitized bronchi
Am J Physiol Lung Cell Mol Physiol, August 1, 2000; 279(2): L209 - L215.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. L. BLACK and P. R. A. JOHNSON
What Determines Asthma Phenotype? Is It the Interaction between Allergy and the Smooth Muscle?
Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): S207 - 210.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. SONG, E. CRIMI, M. MILANESE, J. DUAN, K. REHDER, and V. BRUSASCO
Anti-inflammatory Agents and Allergen-induced beta 2-Receptor Dysfunction in Isolated Human Bronchi
Am. J. Respir. Crit. Care Med., December 1, 1998; 158(6): 1809 - 1814.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1997 American Thoracic Society