Am. J. Respir. Crit. Care Med., Vol 151, No. 6, Jun 1995, 1834-1842.
Experimental hypersensitivity pneumonitis: effect of Thy1.2+ and CD8+ cell depletion
M Schuyler, K Gott, B Edwards and KJ Nikula
Department of Medicine, Albuquerque VA Medical Center, University of New Mexico School of Medicine.
We previously demonstrated that recipient CD4+ cells are necessary for
expression of adoptive murine experimental hypersensitivity pneumonitis
(EHP). In contrast, the acute inflammatory response to intratracheal (i.t.)
administration of Micropolyspora faeni (direct EHP) is not CD4+ cell
dependent (Am. J. Respir. Crit. Care Med. 1994;149:1286-1294). To further
characterize the cells responsible for development of pulmonary
inflammation in recipient animals, we depleted recipients of either Thy1.2+
or CD8+ cells before transfer of M. faeni-sensitized cultured C3H/HeJ
spleen cells and i.t. challenge with M. faeni. We used the same depletion
technique to determine the contribution of these cells to the pulmonary
response to i.t. M. faeni in animals that did not receive cultured cells
(direct EHP). The nature and extent of pulmonary inflammatory changes in
these animals were assayed either 4 d after i.t. challenge with M. faeni in
adoptive EHP or 2 d after i.t. challenge with M. faeni in direct EHP. We
also tested the hypothesis that our previously demonstrated ablation of
adoptive EHP caused by administration of anti-CD4 antibody was due to
depletion of recipient CD4+ cells by allowing recovery of recipient CD4+
cells of anti-CD4- treated animals before i.t. challenge. In addition, we
allowed Thy1.2+ cell recovery of anti-Thy1.2-treated animals before i.t.
challenge. Cultured M. faeni-sensitized spleen cells could adoptively
transfer EHP to animals treated with an irrelevant antibody or PBS.
Depletion of Thy1.2+ but not CD8+ cells ablated the ability of recipient
animals to express adoptive EHP. Direct EHP was not affected by depletion
of Thy1.2+ or CD8+ cells.(ABSTRACT TRUNCATED AT 250 WORDS)
