help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maruyama, N.
Right arrow Articles by Takishima, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maruyama, N.
Right arrow Articles by Takishima, T.

Am. J. Respir. Crit. Care Med., Vol 150, No. 4, Oct 1994, 1086-1093.

Accumulation of basophils and their chemotactic activity in the airways during natural airway narrowing in asthmatic individuals

N Maruyama, G Tamura, T Aizawa, T Ohrui, S Shimura, K Shirato and T Takishima
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.

To investigate cellular differentials in natural airway narrowing of steroid-dependent asthmatic individuals, we performed bronchoalveolar lavage (BAL) on 10 inpatients with asthma treated only with bronchodilators during episodes of natural airway narrowing evaluated by serial monitoring of peak expiratory flow (PEF), and on nine normal volunteers. We confirmed that the airway narrowing was not completely reversed by salbutamol aerosol just before the BAL study, but was completely reversed by administration of systemic steroid after the BAL study. Thus, the natural airway narrowing investigated in this study consisted not only of the constriction of airway smooth muscle, but also of edema of the airway mucosa and/or secretion in airways. Both the numbers and percentages of eosinophils and alcian blue-positive cells in BAL fluids from the asthmatic subjects were significantly higher than those of normals, but the numbers and percentages of neutrophils, lymphocytes, and macrophages were not. Thus, eosinophils and alcian blue-positive cells selectively increased in the airways during the natural airway narrowing. Because we found that the metachromatic cells consisted of two types, with a single nucleus and with segmented nuclei, we further examined basophil chemotactic activity (BCA) in BAL fluids. We showed that BCA was significantly higher in the asthmatic (79.3 +/- 17.2 cells/5 hpf) than in the normal subjects (6.2 +/- 1.6 cells/5 hpf), and also that the activity was more strongly correlated with the cells having segmented nuclei (p = 0.95) than with all of the cells (p = 0.73).(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 J. Immunol.Home page
L. A. Youssef, M. Schuyler, L. Gilmartin, G. Pickett, J. D. J. Bard, C. A. Tarleton, T. Archibeque, C. Qualls, B. S. Wilson, and J. M. Oliver
Histamine Release from the Basophils of Control and Asthmatic Subjects and a Comparison of Gene Expression between "Releaser" and "Nonreleaser" Basophils
J. Immunol., April 1, 2007; 178(7): 4584 - 4594.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. H. K. Lim, M. M. Burdick, S. A. Hudson, F. B. Mustafa, K. Konstantopoulos, and B. S. Bochner
Stimulation of Human Endothelium with IL-3 Induces Selective Basophil Accumulation In Vitro
J. Immunol., May 1, 2006; 176(9): 5346 - 5353.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
B. F. Gibbs, K. E. S. Plath, H. H. Wolff, and J. Grabbe
Regulation of mediator secretion in human basophils by p38 mitogen-activated protein kinase: phosphorylation is sensitive to the effects of phosphatidylinositol 3-kinase inhibitors and calcium mobilization
J. Leukoc. Biol., August 1, 2002; 72(2): 391 - 400.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. L. KEPLEY, P. J. McFEELEY, J. M. OLIVER, and M. F. LIPSCOMB
Immunohistochemical Detection of Human Basophils in Postmortem Cases of Fatal Asthma
Am. J. Respir. Crit. Care Med., September 15, 2001; 164(6): 1053 - 1058.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
G.-J. BRAUNSTAHL, S. E. OVERBEEK, W. J. FOKKENS, A. KLEINJAN, A. R. MCEUEN, A. F. WALLS, H. C. HOOGSTEDEN, and J.-B. PRINS
Segmental Bronchoprovocation in Allergic Rhinitis Patients Affects Mast Cell and Basophil Numbers in Nasal and Bronchial Mucosa
Am. J. Respir. Crit. Care Med., September 1, 2001; 164(5): 858 - 865.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
S. A. Lewis, I. D. Pavord, J. R. Stringer, A. J. Knox, S. T. Weiss, and J. R. Britton
The Relation Between Peripheral Blood Leukocyte Counts and Respiratory Symptoms, Atopy, Lung Function, and Airway Responsiveness in Adults
Chest, January 1, 2001; 119(1): 105 - 114.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Heinemann, A. Hartnell, V. E. L. Stubbs, K. Murakami, D. Soler, G. LaRosa, P. W. Askenase, T. J. Williams, and I. Sabroe
Basophil Responses to Chemokines Are Regulated by Both Sequential and Cooperative Receptor Signaling
J. Immunol., December 15, 2000; 165(12): 7224 - 7233.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
G. M. GAUVREAU, J. M. LEE, R. M. WATSON, A.-M. A. IRANI, L. B. SCHWARTZ, and P. M. O'BYRNE
Increased Numbers of Both Airway Basophils and Mast Cells in Sputum after Allergen Inhalation Challenge of Atopic Asthmatics
Am. J. Respir. Crit. Care Med., May 1, 2000; 161(5): 1473 - 1478.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
O. GHAFFAR, Q. HAMID, P. M. RENZI, Z. ALLAKHVERDI, S. MOLET, J. C. HOGG, S. A. SHORE, A. D. LUSTER, and B. LAMKHIOUED
Constitutive and Cytokine-Stimulated Expression of Eotaxin by Human Airway Smooth Muscle Cells
Am. J. Respir. Crit. Care Med., June 1, 1999; 159(6): 1933 - 1942.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 1994 American Thoracic Society