RJ Dandurand, CG Wang, S Laberge, JG Martin and DH Eidelman
Meakins-Christie Laboratories, Royal Victoria Hospital, Montreal, Quebec, Canada.

The ovalbumin (OA)-sensitized Brown Norway rat (BN) demonstrates early- response (ER) and late-response (LR) allergic bronchoconstriction. To determine whether these responses could be replicated in vitro, we studied lung explants from 8-wk-old male BN rats (wt: 239 +/- 28 g), of which 19 were sensitized to OA (test) and 16 served as controls. Two weeks after sensitization, the animals' lungs were removed, filled with a 1% (wt/vol) agarose-containing solution at 37 degrees C, and cooled to 4 degrees C. Transverse slices (0.5 to 1.0 mm thick) were cut and cultured overnight. Airways were visualized with an inverted microscope and baseline images were obtained with a video camera. To study the ER, 40 airways from 15 test rats and 29 airways from 10 control rats were challenged with 2 micrograms OA and imaged each minute for 10 min. To study the LR, 40 airways from 12 test rats and 44 airways from 12 control rats were challenged with 2 micrograms OA and imaged each hour for 8 h. The maximal response (MR) for each airway was defined as the percent of airway closure. The ER and LR were both defined as an MR > or = mean + 2 SD of the controls. An ER occurred in 38 of 40 test and 2 of 29 control airways (mean MR: 42 +/- 24% versus 4 +/- 3%, p < 0.001), and was completely blocked by methysergide pretreatment in 13 airways.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				H.-P. Hauber, T. Goldmann, E. Vollmer, B. Wollenberg, and P. Zabel Effect of Dexamethasone and ACC on Bacteria-Induced Mucin Expression in Human Airway Mucosa Am. J. Respir. Cell Mol. Biol., November 1, 2007; 37(5): 606 - 616. [Abstract] [Full Text] [PDF]

				M. A. Khan, S. Kianpour, M. R. Stampfli, and L. J. Janssen Kinetics of in vitro bronchoconstriction in an elastolytic mouse model of emphysema Eur. Respir. J., October 1, 2007; 30(4): 691 - 700. [Abstract] [Full Text] [PDF]

				H.-P. Hauber, T. Goldmann, E. Vollmer, B. Wollenberg, H.-L. Hung, R. C. Levitt, and P. Zabel LPS-induced mucin expression in human sinus mucosa can be attenuated by hCLCA inhibitors Innate Immunity, April 1, 2007; 13(2): 109 - 116. [Abstract] [PDF]

				K. S. Kott, K. E. Pinkerton, J. M. Bric, C. G. Plopper, K. P. Avadhanam, and J. P. Joad Methacholine responsiveness of proximal and distal airways of monkeys and rats using videomicrometry J Appl Physiol, March 1, 2002; 92(3): 989 - 996. [Abstract] [Full Text] [PDF]

				A. WOHLSEN, S. UHLIG, and C. MARTIN Immediate Allergic Response in Small Airways Am. J. Respir. Crit. Care Med., May 1, 2001; 163(6): 1462 - 1469. [Abstract] [Full Text]

				L. Cameron, P. Christodoulopoulos, F. Lavigne, Y. Nakamura, D. Eidelman, A. McEuen, A. Walls, J. Tavernier, E. Minshall, R. Moqbel, et al. Evidence for Local Eosinophil Differentiation Within Allergic Nasal Mucosa: Inhibition with Soluble IL-5 Receptor J. Immunol., February 1, 2000; 164(3): 1538 - 1545. [Abstract] [Full Text] [PDF]

				E. M. MINSHALL, R. SCHLEIMER, L. CAMERON, M. MINNICOZZI, R. W. EGAN, J.-C. GUTIERREZ-RAMOS, D. H. EIDELMAN, and Q. HAMID Interleukin-5 Expression in the Bone Marrow of Sensitized Balb/c Mice after Allergen Challenge Am. J. Respir. Crit. Care Med., September 1, 1998; 158(3): 951 - 957. [Abstract] [Full Text] [PDF]