Am. J. Respir. Crit. Care Med., Vol 149, No. 1, 01 1994, 174-177.
Effect of diet on urinary excretion of desmosine and hydroxylysyl pyridinoline
PJ Stone, EC Lucey, GL Snider and C Franzblau
Department of Biochemistry, Boston University School of Medicine, MA 02118.
To help validate the use of urinary desmosine (DES), isodesmosine (IDES),
and hydroxylysyl pyridinoline (HP) as specific markers of host elastin and
collagen degradation, respectively, a study was carried out on the effect
of dietary elastin and collagen on urinary DES, IDES, and HP. Ingestion of
a meal of calf ligamentum nuchae containing 33 g elastin, 500 mg DES, and
400 mg IDES produced a 10-fold increase in urinary DES and an 8-fold
increase in IDES. The urinary DES values remained elevated for more than 10
days following the ingestion. We estimate that about 0.3 mg, or < 0.1%,
of the ingested DES was excreted in the urine. Since ligamentum nuchae is
not a usual ingredient of human diets, we also determined whether a more
typical source and amount of DES, IDES, and HP might affect urinary DES,
IDES, or HP values. Lean ground beef (454 g) was ingested. Our analysis
showed that this meal contained 4 mg DES, 2 mg IDES, and 0.9 mg HP. The
meat-rich diet caused a significant increase of 16 and 34% in the
creatinine and DES content of the urine, respectively. When DES, IDES, and
HP values were normalized for the urine creatinine content, diet had no
effect on the measured amounts. The baseline values (mean +/- SE) for the
volunteers before ingestion of the beef were 8.3 +/- 0.7 micrograms DES/24
h, 8.3 +/- 0.6 micrograms IDES/24 h, and 340 +/- 48 nmol HP/24 h; 5.7 +/-
0.5 micrograms DES/g creatinine, 5.6 +/- 0.4 micrograms IDES/g creatinine,
and 26.9 +/- 2.2 nmol HP/mmol creatinine.(ABSTRACT TRUNCATED AT 250 WORDS)
